T-SPOT.TB在活动性结核与潜伏性结核感染中的诊断价值

Diagnostic Value of T-SPOT.TB in Active Tuberculosis and Latent Tuberculosis Infection

目的:探析T-SPOT.TB在活动性结核与潜伏性结核感染的诊断价值。方法:收集2015年1月~12月某院就诊的疑是活动性结核和潜伏性结核感染患者35例,通过抗酸染色、TB-Ab、TB-PCR、QFT、T-SPOT.TB等方法进行检测,并对活动性结核患者进行诊断和追踪调查,对健康体检者案例进行收录其有无与结核患者接触等情况。结果:结核组的T-SPOT.TB的阳性率要高于非结核组以及健康对照组。活动性结核病诊断所得到的敏感度分别为22%、67%、73%、75%和86%,其中以T-SPOT.TB敏感性最高。结论:T细胞免疫斑点对肺结核的检测具有较高的敏感和特异度,而且这种较高的敏感度与特异度是不受到卡介苗接种与机体免疫功能的影响的。

Objective.To investigate the diagnostic value of T-SPOT.TB in active tuberculosis and latent tuberculosis infection.Methods.35 cases of active tuberculosis and latent tuberculosis infection were collected from January to December in 2015.The patients were diagnosed by anti-acid staining, TB-Ab,TB-PCR, QFT, T-SPOT.TB and other methods.Then carry out the diagnosis and follow-up investigation for active tuberculosis patients as well as record whether the health checkers infect tuberculosis or not.Results：The positive rate of T-SPOT. TB in tuberculosis group was higher than that in non-tuberculosis group and healthy control group.The diagnostic sensitivity of active tuberculosis was 22 %, 67 %, 73 %, 75 % and 86% , respectively. A- mong them, T-SPOT.TB is the most sensitive.Conclusion：T cell immune spots have high sensitivity and specificity to the detection of pulmonary tuberculosis, and this high sensitivity and specificity are not affected by BCG vaccination and immune function.