YAP在氧诱导小鼠视网膜新生血管形成中的表达及意义

Expression and Significance of YAP in Oxygen-Induced Retinal Neovascularization in Mice

目的:研究YAP在氧诱导小鼠视网膜新生血管(retinal neovascularization,RNV)中的表达及意义,探讨YAP对RNV形成的作用。方法:取C57BL/6J小鼠100只随机分为对照组和高氧组,HE染色计数突破视网膜内界膜的新生血管内皮细胞核数,免疫组织化学、Western blot检测YAP蛋白的表达情况。结果:高氧组视网膜可见大片无灌注区和大量突破内界膜的新生血管内皮细胞核(24.65±1.26)个,对照组的无灌注区及新生血管内皮细胞核数(5.12±1.48)明显减少(P<0.05)。高氧组YAP蛋白表达显著增高,有统计学意义(P<0.05)。结论:YAP的异常表达可能与RNV形成密切相关。

Objective：To study the expression and significance of YAP in oxygen-induced retinal neovascularization（RNV）,and to explore the effect of YAP on RNV formation.Methods：100samples of C57BL/6J mice were randomly divided into control group and hyperoxia group.The number of neovascular endothelial cells in the retina was examined by HE staining.The expression of YAP protein was detected by immunohistochemistry and Western blot.Results：There was no perfusion area and a large number of new vascular endothelial cells（24.65±1.26）in the retina of hyperoxia group and no perfusion area and neonatal vascular endothelial cells in the control group（5.12±1.48）was significantly reduced（P〈0.05）.The expression of YAP protein in hyperoxia group was significantly higher than that in control group（P〈0.05）.Conclusion：Abnormal expressionof YAP may be closely related to the formation of RNV.