番茄青枯病植物疫苗胶悬菌剂的制备及其对病害的防治效果

Preparation of colloidal suspension agent used as plant vaccine against tomato bacterial wilt disease and its control efficacy

研究了植物疫苗胶悬剂的制备工艺,确定胶悬剂制备的适宜参数为:琼脂终浓度为2.0‰、氯化钠终浓度为2.0%和pH为7.0。在此条件下,胶悬效果最好,质地均匀,无上下分层。试验了植物疫苗胶悬剂对番茄青枯病的防治效果,结果表明,该胶悬剂能较好地抑制番茄青枯病的发生,其防效(77.45%)与化学农药72%农用硫酸链霉素SP(77.23%)相当,配合生防菌剂20亿活芽孢/g蜡质芽孢杆菌WP施用,效果更佳,防效达81.81%。

The preparation technology of the plant vaccine agent was investigated in this paper.The results showed that the suitable parameters for the plant vaccine agent were 2‰ final concentration of agar,2% final concentration of NaCl and pH7.0.Under this condition,the agent had the best colloidal suspension with uniform texture.The control efficacy against tomato bacterial wilt disease were compared among plant vaccine agent,biocontrol agent 2×10~9 spores/g Bacillus cereus WP and72%streptomycin SP.The results indicated that the control efficacy of plant vaccine agent was 77.45%,equal to that of 72% streptomycin SP（77.23%）.The plant vaccine agent combined with biocontrol agent 2×10~9 spores/g Bacillus cereus WP had the best control effect,with the efficacy of 81.81%.