HLA-A* 0201限制性GPC3表位肽的初步预测与筛选

Prediction and Preliminary Screening of HLA-A* 0201 Restricted Epitope Peptides of Human GPC3

目的:筛选HLA-A*0201限制性GPC3优势表位肽,为肝癌(hepatocellular carcinoma,HCC)特异性的免疫细胞治疗提供新的靶标。方法:通过表位预测网站初步筛选HLA-A*0201限制性GPC3表位肽,T2细胞结合实验筛选优势表位肽。结果:通过三大常用的表位预测网站,共筛选了13个评分较高的表位肽,化学合成表位肽,采用T2细胞结合实验显示,有4个表位肽与HLA-A2呈现较高亲和力。结论:抗原表位预测网站结合T2细胞结合实验能初步筛选亲和力较高的HLA-A*0201限制性GPC3表位肽,可能用于HCC特异性CTL治疗的候选标靶。

Objective To screen the advantage epitope peptides of HLA-A＊0201 restricted human GPC3,so as to provide the new target for hepatocellular carcinoma（ HCC） immunotherapy. Methods Three approved epitope prediction websites were used to predict the epitopes primarily,T2 cells binding assay were performed to screen the optimal epitopes. Results Total 13 epitopes were selected through the three prediction websites,then peptides were synthesized by standard solid phase chemistry. T2 cells binding assay demonstrated that 4 optimal peptides showed high affinity with HLA-A2. Conclusion Combined epitope prediction websites with T2 cells binding assay could accomplish a preliminary screening of HLAA＊0201 restricted epitope peptides of human GPC3,which could be used as optional targets for HCC specific CTLs therapy.