艾灸及艾烟对载脂蛋白E基因敲除小鼠血清MDA、SOD水平的影响

Effects of Moxibustion and Moxa Smoke on Serum MDA and SOD Levels in Apolipoprotein E-deficient Mice

目的:观察艾灸及艾烟对载脂蛋白E基因敲除(Apolipoprotein E-deficient,Apo E^(-/-))小鼠氧化应激相关指标的影响。方法:38只8周龄Apo E^(-/-)小鼠随机分为5组:模型组(n=6)、假艾灸组(n=6)、香烟组(n=8)、艾烟组(n=9)、艾灸组(n=9),13只同龄C57BL/6小鼠作为正常对照,正常组与模型组小鼠暴露于玻璃缸中,艾灸与假艾灸组均对小鼠关元穴进行艾灸,假艾灸组艾条不点燃,艾烟组小鼠暴露于10~15 mg/m3的艾烟环境,香烟组小鼠暴露于10~15 mg/m3的香烟环境,各组小鼠每天干预20 min,连续干预12周,每周干预6 d,于12周末检测小鼠血清氧化应激指标丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)的表达水平。结果:艾灸组及艾烟组血清MDA含量较模型组显著降低(P<0.05),且艾灸组显著低于假艾灸组(P<0.05),艾烟组显著低于香烟组(P<0.05)。艾灸组及艾烟组血清SOD含量较模型组显著升高(P<0.05),艾灸组显著高于假艾灸组(P<0.05),艾烟组显著高于香烟组(P<0.05)。结论:艾灸和艾烟是通过调节机体MDA、SOD水平,发挥抗氧化作用,保护血管内皮改善AS。

Objective：To observe the intervention effects of moxibustion and moxa smoke on oxidative stress indexes in Apo E^-/-mice.Methods：Thirty-eight Apo E^-/-mice（ 8 weeks old） were randomly divided into model group（ n = 6）,sham-moxibustion group（ n = 8）,cigarette smoke group（ n = 8）,moxa smoke group（ n = 9）,and moxibustion group（ n = 9）.Thirteen same age C57 BL /6 mice were used as blank controls.Mice in the blank control and model control groups were put into a glass box without any treatment.Mice of the sham and real moxibustion groups received moxibustion on Guanyuan（ RN4） while the two smoke groups were exposed to smoke,whose concentration is 10 mg / m^3.All the manipulations were performed 20 min / day,6 days each week,12 weeks totally.Levels of malondialdehyde（ MDA） and activity of superoxide dismutase（ SOD） were determined by colorimetric method.Results：Comparing wit the model group,mice in the moxibustion group and moxa smoke group had significant lower level of MDA（ P〈0.05）,and significant higher level of SOD（ P〈0.05）.There was a remarkable decrease of MDA level（ P〈0.05） and an increase of SOD activity（ P〈0.05） in the moxibustion group compared to the sham-moxibustion group.MDA level was decreased（ P〈0.05） and SOD level was significantly increased（ P〈0.05） in moxa smoke group compared to those of the cigarette smoke group.Conclusions：Both moxibustion and moxa smoke can regulate the levels of serum MDA and SOD,which might be one of the mechanisms of their anti-oxidation effects for relieving the damage of vascular intima and atherosclerosis.