艾灸对ApoE^(-/-)动脉粥样硬化小鼠肝脏病理及脂质代谢的作用研究

Effects of Moxibustion on Liver Pathology and Lipid Metabolism in Apolipoprotein E-deficient Mice

目的:观察艾灸对载脂蛋白基因敲除(Apo E^(-/-))动脉粥样硬化(Atherosclerosis,AS)小鼠肝脏病理及血脂相关指标的影响。方法:48只8周龄Apo E^(-/-)小鼠随机分为3组:模型对照组(n=16)、艾灸组(n=16)、西药组(n=16),16只同龄C57BL/6小鼠作为正常对照,固定器固定正常组、模型组小鼠及艾灸组,并对艾灸组小鼠关元穴(RN4)进行艾灸,西药组采用辛伐他汀悬液灌胃(2.8 mg/kg),各组小鼠每天干预20 min,连续干预12周,每周干预6 d,于12周末检测小鼠血清的总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)含量,以油红"O"染色法观察各组小鼠肝脏病理形态变化。结果:各组小鼠血清TC含量呈正常组<西药组<艾灸组<模型组的趋势,艾灸组及西药组血清TG含量较模型组显著降低(P<0.05),西药组、艾灸组小鼠血清LDL含量显著低于模型组(P<0.01;P<0.05),各组小鼠血清HDL含量呈正常组>西药组、艾灸组>模型组的趋势,西药组和艾灸组之间无统计学意义。油红染色可见艾灸组和西药组较模型组肝细胞肿胀程度明显减轻,少量炎细胞浸润及少量红染脂肪滴。结论:艾灸可以降低Apo E^(-/-)小鼠血清TC、TG、LDL含量,升高HDL含量,并对AS过程中的肝脏病理有显著的良性调节作用,可以通过调节脂代谢的途径,改善AS。

Objective：To observe the intervention effects of moxibustion on liver pathology and lipid metabolism in Apo E^-/-mice with atherosclerosis.Methods：Forty-eight Apo E^-/-mice（ 8 weeks old） were randomly divided into model group（ n = 16）,moxibustion group（ n = 16）,and medicine group（ n = 16）.Sixteen same age C57 BL /6 mice were used as blank controls.Mice in the blank control and model control group were fixed without any treatment.Mice of the moxibustion groups received both fixation and moxibustion on Guanyuan（ RN4）.Medicine group received simvastatin suspension（ 2.8 mg / kg） by intragastric administration.All the manipulations were given 20 min each day,6 days each week,12 weeks totally.Contents of total cholesterol（ TC）,triglyceride（ TG） and low density lipoprotein cholesterol（ LDL-C） and high-density lipoprotein cholesterol（ HLD-C） were determined by colorimetric method.Liver pathological changes of mice in each group were observed by oil red ＂O＂staining method.Results：TC content of mice in each group was normal group 〈medicine group 〈moxibustion group model group.Comparing to the model group,mice in the moxibustion group and medicine group has significant lower level of TG（ P〈0.05） and LDL（ P〈0.05）.The content of serum HDL showed：normal group〉 medicine group,moxibustion group〉 model group.There was no significant differences between medicine group and moxibustion group.There was a remarkable decreasing of swelling degree of liver cells and less infiltration of inflammatory cells and fewer red-stained fat droplets in the moxibustion group and medicine group compared with the model group.Conclusion：Moxibustion can decrease the content of serum TC,TG,LDL and increase the content of HDL in Apo E^-/-mice,and significantly regulate the liver pathology in the process of AS,which may be one of the mechanisms for relieving the damage of vascular intima and atherosclerosis by regulating lipid metabolism.