维药黑种草子对急性乙醇性肝损伤小鼠保护作用及抑制NF-κB炎性反应信号通路的研究

Study on the Protective Effect of Uygur Medicine Nigellae Semenon on Acute Alcoholic Liver Injury Mice and its Inhibition on NF-кB Signaling Pathway

目的:观察维药黑种草子对急性乙醇性肝损伤小鼠保护作用及对NF-κB炎性反应信号通路的抑制作用。方法:50只昆明小鼠随机分为模型组、空白组、阳性对照组、黑种草子水提液高剂量组(6 g/kg)、黑种草子水提液低剂量组(2 g/kg)。除空白组外,其余各组每日均灌胃给予56度白酒10 m L/kg,连续灌胃15 d。禁食不禁水10 h后,观察小鼠一般状态,检测体质量,肝重,肝指数,肝功能指标谷丙转氨酶(ALT)、谷草转氨酶(AST),肝组织核因子κB(NF-κB)及肿瘤坏死因子-α(TNF-α)的表达,并进行肝组织病理学检查。结果:与空白组相比,模型组小鼠体质量和肝指数均降低,血清ALT及AST活性升高,差异有统计学意义(P<0.05)。与模型组相比,黑种草子水提液各组小鼠肝体质量和肝指数有所升高,血清ALT及AST活性降低,差异有统计学意义(P<0.05)。模型组小鼠肝细胞明显气球样变性、坏死和并伴有少量炎细胞浸润,黑种草子水提液组较肝损伤模型组病变情况显著减轻。模型组NF-κB及TNF-α表达强度均高于空白组,黑种草子各给药组较模型组NF-κB及TNF-α表达强度均下降。结论:黑种草子水提液对急性乙醇性肝损伤小鼠具有一定保护作用,并且能明显抑制小鼠肝细胞NF-κB及TNF-α的表达。

Objective： To explore the protective effects of Uygur medicine Nigellae Semen on acute alcoholic liver damage mice and its inhibition on NF-кB signaling pathway. Methods： After establishing acute alcoholic liver injury mice model,50 mice were randomly divided into blank group,model group,positive control group,nigellae semen extraction high dose（ 6 g / kg） group,and nigellae semen extraction low dose（ 2 g / kg） group. Except for the blank group,the other groups were orally administered 56 degree liquor 10 m L / kg for 15 days. After 10 hrs of food forbidden but no water forbidden,observe the general state of mice,detect their body weight,liver weight,liver index,liver function indexes of alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,the expression of NF-кB and TNF-α in liver tissue and liver tissue pathological changes. Results： Compared with the blank group,the body weight and liver index of the model group decreased,and serum ALT and AST activity significantly increased（ P〈0. 05）.Compared with the model group,the liver weight of mice,body weight and liver index increased in nigellae semen groups,while serum ALT and AST activity decreased significantly（ P〈0. 05）. Model group mice showed significantly hepatocytes ballooning degeneration,necrosis and accompanied by a small amount of inflammatory cells infiltration. Compared with the model group,the nigellae semen group showed lower pathological changes. Compared with the blank group,the serum concentrations of NF-кB and serum TNF-α content significantly increased in model group. Compare with the model group,the serum concentrations of NF-кB and serum TNF-α content significantly decreased in the nigellae semen groups. Conclusion： Nigellae semen extraction has a protective effect on acute alcoholic liver damage mice,and it can inhibit the expression of NF-кB and TNF-α liver cells.