益消方对自发糖尿病小鼠血清和心肌组织IGF-1表达的影响

Effect of Yi Xiao Decoction on IGF-1 in Mice of Diabetic Myocardial Disease

目的:观察益消方对KKAy糖尿病小鼠血清和心肌组织IGF-1表达的影响,探讨其对糖尿病心肌保护的可能机制。方法:选用KKAy小鼠及C57BL/6J小鼠,随机分为正常组、模型组和中药组,分别灌服蒸馏水及益消方中药,在实验的4、8、12周取材后,运用ELISA的方法检测血清中IGF-1的表达,运用实时定量PCR的方法检测心肌组织IGF-1 mRNA的表达。结果:在实验的不同阶段,与正常组相比,KKAy糖尿病小鼠模型组的血清及心肌组织中IGF-1的表达明显降低(P<0.01),而与模型组相比,中药组的血清IGF-1在第8周和第12周表达明显升高(P<0.05)。心肌组织的IGF-1 mRNA在第12周表达有升高的趋势。结论:益消方可通过改善血清及心肌组织中IGF-1的表达,从而对自发糖尿病小鼠的心肌具有一定的保护作用。

Objective： To study the protective effect of＂Yi Xiao Decoction＂on serum and myocardial tissue of KKAy mice model,and discuss the possible mechanisms of its diabetic myocardial protection. Methods： The KKAy and C57 BL /6J mice were randomly divided into the model group,normal group and the TCM group. The mice in different groups were administered distilled water and Yi Xiao Decoction,respectively by gavage. Specifically,the TCM group was fed with Yi Xiao Decoction,while the model group and the normal group were given distilled water. The expression of IGF-1 in serum was detected by ELISA,and expression of IGF-1 mRNA expressions in myocardia by RT-PCR after 4,8,12 weeks＇ treatment. Results： In the different stages of the experiment,compared with the normal group,the expression of IGF-1 in serum and tissues in diabetic KKAy mice decreased significantly in the model group（ P〈0. 01）,and compared with the model group,the expression of serum IGF-1 in TCM group in 8th weeks and 12 th weeks increased significantly（ P〈0. 05）. The expression of mRNA IGF-1 in cardiac muscle tissue increased in 12 th weeks. Conclusion： Yi Xiao Decoction can improve IGF-1 expression in serum and myocardial tissues and protect cardiac muscle tissue of the diabetic mice model.