5味山姜属中药对胃溃疡寒证大鼠环核苷酸水平的影响

Effect of Five Alpinia Roxb to Model Rats with Cold Stomach Syndrome in cAMP,cGMP,cAMP/cGMP Level

目的:研究大高良姜、高良姜、益智、草豆蔻、红豆蔻5味山姜属中药对胃溃疡寒证大鼠c AMP、c GMP含量的影响。方法:分空白组、模型组、附子理中丸阳性对照组、5味受试药物高低剂量组,采用15%冰乙酸加4℃知母水煎液造胃溃疡寒证模型。造模成功后给药,采用ELISA法测定血浆、血小板、血管平滑肌、胃组织c AMP、c GMP含量,计算c AMP/c GMP比值。结果:与模型组比较,除血小板益智低剂量组外,其余9个受试组血浆、血小板、血管平滑肌、胃组织c AMP含量均升高(P<0.01或P<0.05);除血小板益智低剂量组,胃组织草豆蔻、红豆蔻、益智低剂量组外,其余受试组血浆、血小板、血管平滑肌、胃组织的c AMP/c GMP比值明显增高(P<0.01或P<0.05)。各受试组c GMP含量差异均无统计学意义。结论:5味山姜属中药能显著升高胃溃疡寒证大鼠血浆、血小板、血管平滑肌、胃组织c AMP含量及c AMP/c GMP比值,这可能是5味山姜属中药温热药性和温胃散寒作用的科学内涵。

Objective：To study the effect of the five Alpinia Rox（Alpinia officinarum Hance, Alpinia galangal（L.）willed, Alpin-ia katsumadai Hayata, Alpinia galangal, Alpinia oxyphyllaMiq ） on cyclie Adenosine monnphosphate （ cAMP） levels, cyclie guanosine monophosphate （ cGMP） levels, and ratio of cAMP/cGMP in stomach excessive cold syndrome rats .Methods：Rats were divided into blank control group , model group , 2 positive control group , and 10 warm-heat herb groups , with 10 rats in each group.Rats model of stomach excess-cold syndrome was established by intragastric infusion of the 15% glacial acetic acid and 4℃ Common Anemarrhena Rhizome decoction liquid .After establishing the model , blank group and model group were given water , warm-heat herb respectively .The contents of cAMP , cGMP of stomach tissue , serum, vascular smooth muscle platelet were detec-ted by the method of enzyme-linked immunoassay .Meanwhile , the cAMP/cGMP ratio was calculated .Results： Compared with model group, the cAMP level of 10 subjects content in rat stomach tissue , serum,thrombocyte and vascular smooth muscle were higher （P〈0.01,P〈0.05）;no obvious difference in cGMP content;except for low doses in Alpinia katsumadai , Galanga Galan-gal Seed and Alpinia oxyphylla , ratio of cAMP/cGMP in rest drug participants increased obviously （P〈0.01,P〈0.05）.Con-clusion：Five Alpinia Roxb may elevate levels of cAMP , cAMP/cGMP ratio in model rats with stomach excessive cold syndrome , which may reveal the fact that five Alpinia Roxb have the effect of warming stomach for dispelling cold .