天麻素对高血压大鼠血压变化、血管保护作用及氧化应激反应机制研究

Gastrodin's Effect on Rats Blood Pressure Changes and Vascular Protective Effects of Oxidative Stress Response

目的:探讨天麻素对高血压大鼠血压变化、血管保护作用及氧化应激反应机制研究。方法:50只大鼠随机分为正常对照组、模型组、替米沙坦组、天麻素低剂量组、天麻素高剂量组,每组大鼠10只。正常对照组给予等剂量生理盐水灌胃,模型组于造模成功后给予等剂量生理盐水灌胃,替米沙坦组于造模成功后给予5 mg/(kg·d)灌胃,天麻素低剂量组于造模成功后给予40 mg/(kg·d)灌胃,天麻素高剂量组于造模成功后给予160 mg/(kg·d)灌胃。各组大鼠均连续灌胃8周,1次/d。结果:与模型组比较,替米沙坦组和天麻素各剂量组收缩压均下降(P<0.05);与替米沙坦组比较,天麻素高剂量组收缩压下降(P<0.05),天麻素低剂量组收缩压上升(P<0.05);天麻素高剂量组收缩压明显低于天麻素低剂量组(P<0.05);与模型组比较,替米沙坦组和天麻素各剂量组ET-1含量均下降,而NO含量均上升(P<0.05);与替米沙坦组比较,天麻素高剂量组ET-1含量下降及NO含量上升(P<0.05),而天麻素低剂量组ET-1含量上升及NO含量下降(P<0.05);天麻素高剂量组ET-1含量低于天麻素低剂量组,而NO含量高于天麻素低剂量组(P<0.05);与模型组比较,替米沙坦组和天麻素各剂量组SOD和MDA含量均下降(P<0.05);与替米沙坦组比较,天麻素高剂量组SOD和MDA含量下降(P<0.05);天麻素高剂量组SOD和MDA含量低于天麻素低剂量组(P<0.05)。结论:天麻素可明显降低高血压大鼠血压,且可达到血管保护作用及抗脂质过氧化损伤,故而值得进一步研究。

Objective：To investigate the gastrodin′s effect on blood pressure ,vascular protective effect and mechanism of oxidative stress in hypertensive rats .Methods：A total of 50 rats were randomly divided into normal control group ,model group ,telmisartan group,gastrodin low dose group,high dose group,10 rats in each group.The normal control group were given the same dose of nor-mal saline,model group given dose of normal saline after modeling ,telmisartan group 5 mg/（kg-D）by gavage after modeling,gast-rodin low dose group 40 mg/（ kg-D） by gavage ,high dose group 160 mg/（ kg-D） by gavage .Rats in each group were administered o-rally for 8 weeks,once a day .Results：Compared with the model group ,systolic blood pressure of telmisartan group and gastrodin in each dose group dropped（P〈0.05）;compared with telmisartan group,gastrodin high-dose group′s systolic blood pressure rose（P〈0.05）,gastrodin low-dose group′s systolic blood pressure dropped （P〈0.05）;gastrodin high dose group′s systolic pressure was significantly lower than that of the low dose group （ P〈0.05 ）; compared with the model group , telmisartan group and gastrodin group′s ET-1 content dropped,while the content of NO increased（P〈0.05）;compared with telmisartan group,high dose group′s content of ET-1 dropped and the content of NO increased （P〈0.05）,and the gastrodin low dose group ET-1 content increased and the content of NO dropped （ P〈0.05）;gastrodin high dose group′s ET-1 was lower than that of low dose group ,and NO content was higher than the low dose group（P〈0.05）;compared with the model group,SOD and MDA of telmisartan group and gastrodin each dose group dropped（P〈0.05）; compared with telmisartan group,SOD and MDA dropped in high dose group（P〈0.05）;the content of SOD and MDA of gastrodin in high dose group were lower than that of gastrodin low dose group （P〈0.05）.Conclu-sion：After using gastrodin ,the blood pressure of hypertensive rats can be dropped obviously ,and the protective effect of the blood vessel and the anti lipid peroxidation damage can be achieved ,which is worth further study .