从神经肽类相关物质中的实验研究探究脏腑相关的理论内涵

An Experimental Study of “Interconnection of Zang-fu Viscera”Theory:Based on Neuropeptide Correlative Substances

目的：以中医脏腑相关理论为指导思想,通过建立肺病动物模型与肠病动物的模型,分别选取2个不同时间点（8d、16 d）观察12类不同的组织中相关活性物质SP、VIP、i NOS、CGRP的表达与关联,探求脏腑相关的病理实质。方法：本实验采用2批健康雌性SD大鼠,每只大鼠体重在110~120 g之间,每批35只。随机将每批的35只大鼠分为5组,均为正常组、限食组、限水组、高氧组和低氧组,测定肺、脾、肝、膀胱、肾脏、血清、胃、心脏、空肠、回肠、结肠、直肠中CGRP、i NOS、SP和VIP等物质的含量的变化来观察各个脏腑在神经肽相关物质的相关联系。结果：在CGRP这个指标有关联的组织有：肺和心脏（8 d）、肺和空肠（16 d）、肾和膀胱（8 d）、脾与肝（16 d）、心与回肠（16 d）;在i NOS这个指标有关联的组织有：肺与膀胱（16 d）、心与直肠（16 d）;在SP这个指标有关联的组织有：肺与肝（8 d和16 d）、肺与回肠、结肠（8 d）、脾与胃（8 d）、心脏和回肠（8 d）;在VIP这个指标有关联的组织有：肺和肝（8 d）、心脏和结肠（8 d和16 d）。结论：通过论证肺与大肠、肝脏、膀胱、心脏的关系,丰富了＂肺与大肠相表里＂的观点,对临床治疗有指导意义。此外,通过CGRP、SP、i NOS、VIP等指标发现心脏与肠道之间也有密切联系。

Objective：To explore the pathology of “interconnection of zang-fu viscera” theory with the guide of it , building pulmo-nary disease animal models and intestinal disease ones and observing the expression and correlation of SP , VIP, iNOS and CGRP in 12 different types of tissue at 12 various chosen time （8 d, 16 d） .Methods：Two suites of healthy female SD rats were adopted , 35 rates per suite, and the body weight of each rat is between 110 to 120 g.Every suite was randomized into 5 groups： normal group, food restriction group, water limiting group, high oxygen group and low oxygen group, and the content of CGRP, iNOS, SP and VIP in the lung, spleen, liver, bladder, kidney, serum, stomach, heart, jejunum, ileum, colon, rectum were tested.Re-sults：The tissues related to CGRP were：the lung and the heart（8 d）,the lung and the jejunum（16 d）, the kidney and the the bladder （8 d）, the spleen and the liver （16 d）, the heart and the ileum （16 d）;The tissues related to iNOS were：the lung and the bladder （16 d）, the heart and the rectum （16 d）;The tissues related to SP were：the lung and the liver （8 d and 16 d）, the lung, the colon and the ileum（8 d）, the spleen and the stomach （8 d）, the heart and the ileum （8 d）;The tissues related to VIP were：the lung and the liver （8 d）, the heart and the colon （8 d and 16 d）.Conclusion：By illustrating the relationship between the lung and the large intestine , liver, bladder and heart , the theory “the lung and the large intestine being interior-exteriorly re-lated” were enriched, which had great guiding significance to clinical treatment .In addition, CGRP, SP, iNOS, VIP and other indicators showed a close relationship between the heart and the intestine .