HPLC法测定芪红水煎剂中花旗松素和槲皮素的含量

Determination of Taxifolin and Quercetin in Qihong Decoction:HPLC Method

目的：建立高效液相色谱法测定芪红水煎剂中2种主要有效成分花旗松素和槲皮素的含量。方法：采用Waters Sunfire C（18）（250 mm×4.6 mm,5μm）色谱柱,以甲醇-0.5%磷酸溶液为流动相梯度洗脱,流速1 m L/min,检测波长290 nm（花旗松素）、365 nm（槲皮素）。结果：花旗松素和槲皮素线性范围分别为0.075 5~0.120 8μg（r=0.999 4）和0.030 8~0.092 4μg（r=0.999 9）;平均加样回收率（n=6）分别为102.74%（RSD=1.71%）和102.45%（RSD=1.52%）。结论：本法简便、准确,重复性好,可用于芪红水煎剂中花旗松素和槲皮素的含量测定。亦可作为芪红水煎液质量控制方法之一。

Objective：To establish an HPLC method for the determination of taxifolin and quercetin in Qihong decoction .Meth-ods：The determination was carried out with Waters Sunfire C 18 column （250 mm ×4.6 mm, 5μm）, using methanol-0.5%phos-phoric acid as mobile phase with gradient elution at a flow rate of 1.0 mL/min and it was detected that the wave length was 290 nm （taxifolin） and 365 nm （quercetin）.Results：The linear ranges of taxifolin and quercetin were 0.0755~0.1208 μg（r=0.9994） and 0.0308~0.0924 μg（r=0.9999）;the average recovery rate （n=6） was 102.74%（RSD=1.71%） and 102.45% （RSD=1.52%） respectively .Conclusion：This method was simple , steady and reliable , which could be used to determine the contents of taxifolin and quercetin and to control the quality of Qihong decoction .