艾灸足三里脊髓响应通路的实验研究

Moxibustion in Conduction Response Pathway between Zusanli and Spinal Cord

目的:研究艾灸"以温促通"的传入通路与脊髓背角神经元响应的关系。方法:选用成年雄性SD大鼠40只,玻璃微电极细胞外记录L_4节段脊髓背角神经元活动(放电频率),分别给予足三里肌注150μL的0.01M辣椒素(Capsaicin,CAP)和49℃艾灸30 s刺激,观察不同刺激后神经元放电的变化。结果:1)脊髓背角神经元对足三里注射辣椒素有响应,其效应特征以兴奋性作用为主;2)49℃艾灸足三里穴与辣椒素穴位注射足三里可以在脊髓背角的同一个神经元中汇聚;3)脊髓背角神经元对49℃艾灸的响应,也存在与足三里辣椒素注射不一致效应。结论:高温热灸的传入通路和脊髓响应,部分是由辣椒素敏感的C纤维和TRPV1通道介导的。

Objective：To study the relationship between the afferent pathway of moxibustion and the response of dorsal horn neu-rons of the spinal cord,warming the meridians to eliminate stagnation.Methods：There were 40 Adult male SD rats selected,extracellular recording the activity of spinal dorsal horn neurons of L4 by glass microelectrode （discharge frequency）,given intramuscular injection of 150 μl 0.01Mcapsaicin at Zusanli and at 49 degrees for 30 s to observe the changes of different neuron discharge before and after different stimulation.Results：1）Spinal dorsal horn neurons gave response to capsaicin stimulation,which was characterized by excitatory effect.2）49 degrees of moxibustion at Zusanli point and capsaicin injection could converge on the same neurons in the dorsal horn of spinal cord.3）The response of spinal dorsal horn neurons to the 49 degrees of moxibustion were inconsistent with that of capsaicin injection at Zusanli.Conclusion：The afferent pathway and the spinal cord response with high temperature moxibustion were mediated by capsaicin sensitive C fibers and TRPV1 channels partially.