维吾尔族药阿纳其根中绿原酸的含量测定

Method Study for Determination of Chlorogenic Acid in Anacyclus pyrethrum DC. of Uygur Medicine by RP-HPLC

目的:建立测定维吾尔族药阿纳其根中绿原酸含量的高效液相色谱法(HPLC)。方法:色谱柱为Agilent 5 TC-C_(18)柱(5μm,4.6 mm×150 mm),以乙腈-0.4%磷酸水(13∶87,V/V)为流动相,流速为0.8 m L·min^(-1),紫外检测波长为327nm。结果:绿原酸在质量为0.0484 mg^0.968 mg范围内呈良好线性关系,回归方程:Y=2296316.8X-1369.4(r=0.9999),平均回收率为100.20%,RSD=2.75%。结论:该方法简便、灵敏、重现性好,可用于阿纳其根中绿原酸的含量测定。

Objective：A high performance liquid chromatography method（HPLC）was established to determine chlorogenic acid in the root of Anaycclus pyerhturm.DC.Methods：HPLC ana1ysis was actualized on an Agilent 5 TC-C18 （5 μm,4.6 mm ×150 mm） column with mobile phase of acetonitrile -0.4% phosphoric acid solution（13∶87,V /V）,flow rate was 0.8 mL·min^ -1 ,detection wavelength was 327 nm.Results：The calibration curve was a good linear with a range of 0.0484 -0.968 m·for chlorogenic acid （Y =2296316.8X-1369.4,r =0.9999 ）.The average recovery rate for chlorogenic acid was 100.20%,RSD =2.75%.Conclusion：This method is simple,sensitive,reproducible,which is suitable for the quality control of the root of Anaycclus pyerhturm.DC.