葛根药材中化学成分HPLC指纹图谱研究

Study on HPLC Fingerprint Analysis on Chemical Composition of Radix Puerariae

目的:探索研究建立葛根高效液相指纹图谱的方法与条件,以更好地控制葛根质量。方法:Agilent1260高效液相色谱仪;kromasil 100-5C18(4.6 mm×250 mm,E63848,25 cm)色谱柱;流动相:A-乙腈;B-0.1%磷酸水溶液,梯度洗脱,流速为1 m L/min,柱温为30℃,检测波长为250 nm,探索研究了葛根药材以及提取物指纹图谱。结果:运用梯度洗脱法得到的色谱图各色谱峰分离较好,达到指纹图谱要求,初步建立了葛根的指纹图谱。结论:HPLC指纹图谱法使用方法不同,所得到的图谱结果也会有所不同,可根据最后所得到的理想指纹图谱来断定高效液相的最佳使用条件方法。

Objective：To study the method and condition for HPLC fingerprinting of Radix Puerariae,in order to better control the quality of herbal medicine.Methods：Agilent 1260 by high performance liquid chromatograph was applied and kromasil 100-5C18 （4.6 mmx250 mm,E63848,25 cm）chromatographic column was established.The mobile phase was A-acetonitrile and B-0.1%phosphoric acid aqueous solution with gradient elution and velocity was set as 1 mL/min with the column temperature for 30 ℃. The detection wavelength was 250 nm to explore and study Radix Puerariae extracts of medicinal herbs as well as fingerprints.Re-sults：Separation in each chromatographic peak of obtained chromatogram by the above method was great,meeting the requirements to fingerprints.The fingerprint for Radix Puerariae was preliminary established.Conclusion：As different HPLC fingerprint meth-ods are applied,the map results will vary.The optimal use and ideal conditions of high performance liquid chromatography method can be summarized based on the result of the best fingerprints.