Preparation of Intelligent Supramolecular Hydrogels and Its application as 3D culture scaffold of ovarian cancer cell

Destination:to prepare intelligent suparmolecular hydrogel by mimicking the key features of extracellular matrices(ECM)and culture ovarian cancer cells in 3D mold,then investigate the influence of microenvironments on behavior of cells.Method:Through using the inclusion complexes interact between trans-azobenzene(or adaman-

Destination： to prepare intelligent suparmolec- ular hydrogel by mimicking the key features of extra- cellular matrices （ECM） and culture ovarian cancer cells in 3D mold, then investigate the influence of microenvironments on behavior of ceils. Method：Through using the inclusion complex- es interact between trans-azobenzene （or adaman- tane） groups and cyclodextrin cavities, some novel supramolecular hydrogels based on poly（methyl vi- nyl ether-alt-maleic acid） （P（MVE-alt-MA）） were formed from azobenzene （or adamantane） grafted P（MVE-alt-MA） （P（MVE-alt-MA）-g-Azo （or Ad）） （guest polymer） and β-cyclodextrin grafted P（MVE-alt-MA） （P（MVE-ah-MA）-g-β-CD） （host polymer） respectively. These hydrogels were used as matrix to culture ovarian cancer cells.