刺萼龙葵土壤微生物区系及相关功能菌研究

Study on microbial flora and functional bacteria fromSolanum rostratumsoil

选取吉林省白城市洮北区五个样区,进行选择性采土及抽样调查。利用平板法对采集刺萼龙葵根际土壤进行微生物培养。通过对细菌、放线菌、真菌、功能菌的培养及菌落测定,得出不同地域生长刺萼龙葵的土壤中细菌、放线菌和真菌的菌落数随着光照、人流量的增加而增加,随着土壤水分的减少而增加。检测发现生长刺萼龙葵的土壤中确实存在亚硝酸细菌、反硝化细菌,经分析刺萼龙葵并没有对亚硝酸细菌和反硝化细菌产生影响。不同地域各组间没有差异显著性,可能与刺萼龙葵的生长环境和自身特点有关。革兰氏阴性菌和革兰氏阳性菌不符合1∶1的比例。

Five sites were selected in Baicheng city for taking soil samples and conducting the experiment.Microorganisms with rhizosphere soil where Solanum rostratum grows were cultivated,by using plate method,and then,quantities of bacteria,Actinomyces,Fungi and functional bacteria colonies were tested.The results show that quantities of bacteria,Actinomyces,Fungi in different growing area increase with the sunshine and people flow,and with the reducing of water content.Therefore,nitrite bacteria and denitrifying bacteria exist in rhizosphere soil,and the growing of Solanum rostratum has no effect on the two bacteria.Related to the growing conditions and characteristics of Solanum rostratum,there are no significant differences within 5sample sites.The negative and positive Gram stain results don＇t live up to 1∶1.