Lgr5、VEGF及VEGFR-2在结直肠癌中的表达及其临床意义

Study on expressions of Lgr5,VEGF and VEGFR-2 in colorectal carcinoma and its clinical significance

目的探讨富含亮氨酸重复序列G-蛋白耦联受体(Lgr5)、血管内皮生长因子(VEGF)及血管内皮生长因子受体-2(VEGFR-2)在结直肠癌和正常结直肠黏膜组织中的表达情况及与结直肠癌临床病理特征和预后的关系。方法选取90例结直肠癌患者癌组织标本作为观察组,选取其中10例患者癌旁正常结直肠黏膜组织标本作为对照组,应用免疫组化法检测2组标本中Lgr5、VEGF及VEGFR-2蛋白表达情况,分析Lgr5、VEGF及VEGFR-2表达与结直肠癌患者临床病理特征与预后的关系。结果观察组Lgr5、VEGF及VEGFR-2阳性表达率均明显高于对照组(P均<0.05)。观察组中Lgr5、VEGF及VEGFR-2阳性表达率与分化程度、有无淋巴结转移、临床分期、肿瘤大小、浸润深度均有明显相关性(P均<0.05),且线性相关性分析显示Lgr5、VEGF及VEGFR-2之间呈正相关性(P均<0.05);生存分析显示Lgr5、VEGF及VEGFR-2表达均与患者的预后相关(P均<0.05)。结论结直肠癌患者癌组织中Lgr5、VEGF及VEGFR-2高表达且具有协同正向作用,共同促进肿瘤的形成和进展。

Objective It is to observe the expressions of Lgr5, VEGF and VEGFR-2 in colorectal cancer and normal color- eetal mucosa, and investigate their relationship with the clinicopathological features and prognosis of patients with colorectal eaneer. Methods 90 cases of colorectal adenocarcinoma tissue specimens as observation group and 10 cases of normal eolorec- tal mucosa as control control group were collected. The expressions of Lgr5, VEGF and VEGFR-2 protein were detected by im- munohistochemical method in the two groups. The relationship of the expression of Lgr5, VEGF, and VEGFR-2 with elinico- pathological teatures and prognosis in patients with colorectal cancer were analyzed. Results The positive rate of Lgr5, VEGF and VECFR-2 expression in the observation group were significantly higher than those in the control group （ all P 〈 0.05 ）. The positive rate of Lgr5, VECF and VEGFR-2 expression were significantly correlated with differentiation, lymph node metas- tases, clinical stage, diameter, and muscle layer invasion in observation group （ all P 〈 0.05） , and the positive correlations between Lgr5, VEGF and VEGFR-2 were found in the observation group by linear analysis （ all P 〈 0. 05 ）. The expressions of Lgr5, VEGF and VEGFR-2 were eorrelated with the prognosis of patients by survival analysis （ all P 〈 0.05 ）. Conclusion The higher expressions of Lgr5, VEGF and VEGFR-2 can promote the occurrence and development in eolorectal cancer.