泄浊解毒方对溃疡性结肠炎大鼠血清TLR4、MD2、IL-13及NF-κB的影响

Effect of Xiezhuojiedu decoction on TLR4,MD2,IL-13,NF-κB on ulcerative colitis model rats

目的观察泄浊解毒方对溃疡性结肠炎(UC)大鼠血清Toll样受体4(TLR4)、髓样分化蛋白-2(MD2)、白介素-13(IL-13)及结肠组织核因子kB(NF-κB)的影响。方法采用三硝基苯磺酸(TNBS)/乙醇联合造模法诱导大鼠溃疡性结肠炎动物模型。雄性Wistar大鼠45只随机抽取8只为空白对照组,将造模成功的大鼠分为模型组、柳氮磺吡啶组、泄浊解毒方高剂量组、泄浊解毒方低剂量组,每组8只。空白对照组正常喂养,模型组予生理盐水灌胃,柳氮磺吡啶组予SASP按0.3 g/kg体质量灌胃,泄浊解毒方高剂量组、泄浊解毒方低剂量组分别予泄浊解毒方颗粒10.0 g/kg、5.0 g/kg灌胃。各灌胃组大鼠每日给药1次,连续14天。检测各组大鼠血清TLR4、MD2、IL-13及NF-κB含量。结果泄浊解毒方高剂量组能明显降低TLR4、MD2、NF-κB含量,升高IL-13含量,组间比较差异有统计学意义(F=110.490、271.137、10.723、92.297,P<0.05)。结论泄浊解毒方对UC大鼠治疗效果显著,其作用机制可能与降低TLR4、MD2、NF-κB含量,升高IL-13含量有关。

Objective To observe the effects of Xiezhuojiedu decoction on TLR4, MD2, IL-β and NF-κB of ulcera- tive colitis（UC） rats . Methods Established the animal modds of ulcerative colitis with TNBS/ethanol joint modeling meth- od. After the irrigation treatment, the expression of TLR4, the content of MD2, IL-β and NF-κB in serum were detected, respectively. Results Xiezhuojiedu decoction can decrease the expression of TLRg, MD2 and NF-κB,improve the level of IL- β. The comparison between the two groups showed statistical significance （ F = 110. 490, F = 271. 137, F = 10.723, F = 92.297, P 〈 0.05 ） . Conclusion The effects of Xiezhuojiedu decoction on UC rats are remarkable, it may be related to de- creasing the expression of TLR4, MD2, and NF-κB,improving the level of IL-β.