甘草酸二胺对腹膜透析模型大鼠腹膜功能的保护作用研究

Protective effects of diammonium glycyrrhizinate on peritoneal dialysis rat model of peritoneal function

目的研究甘草酸二胺对腹膜透析模型大鼠腹膜功能保护作用机制。方法将清洁级雄性大鼠40只随机分成4组,分别为空白对照组、模型组、己酮可可碱组、甘草酸二胺组,每组10只,连续实验4周,4周后观察大鼠腹膜功能(超滤量、Durea/Purea)和相关指标变化(尿素氮、肌酐及清除率、TNF-α、MCP-1)。结果模型组超滤量、D4/DO较己酮可可碱组、甘草酸二胺组降低,Durea/Purea升高(F=8.935、7.824、9.361,P<0.05),己酮可可碱组较甘草酸二胺组超滤量、Durea/Purea、D4/D0降低,但2组比较差异不显著(P>0.05)。与模型组比较,己酮可可碱组、甘草酸二胺组尿素氮、肌肝清除率升高(F=7.363、8.923,P<0.05),己酮可可碱组较甘草酸二胺组尿素氮、肌酐清除率升高,但2组比较差异不显著(P>0.05);与模型组比较己酮可可碱组、甘草酸二胺组TNF-α、MCP-1降低(F=7.824、7.911,P<0.05),甘草酸二胺组较己酮可可碱组TNF-α、MCP-1降低,2组比较差异不显著(P>0.05)。结论 4.25%葡萄糖腹膜透析液能使大鼠腹膜功能受损,甘草酸二胺能减轻腹膜损伤,降低腹膜功能恶化。

Objective To study the protective effects of diammonium glycyrrhizinate on the peritoneal function of per- itoneal dialysis rats. Methods Forty male rats were randomly divided into five groups, namely control group, model group, saline group, pentoxifylline group, glycyrrhizin diamine group, 10 rats in each group, continuous experiment for 4 weeks, the peritoneal function in rats after 4 weeks （ ultrafiltration, Durea/Purea） and related indexes （ blood urea nitrogen creatinine clearance rate, TNF-α, and MCP-1 ） were tested. Results The amount of ultrafiltration, D4/D0 of model group compared with pentoxifylline group and glycyrrhizin diamine group reduced significantly, Durea / Purea increased significantly （ F = 8. 935,7. 824,9. 361, P 〈 0.05 ）, pentoxifylline group＇s ultrafihration volume increased significantly than in the glycyrrhiz- inate diamine group, while Durea / Purea, D4/D0 were more lower, but the difference were not significant （ F = 7. 363; 8. 923, P 〉 0.05 ）. Model groupg urea nitrogen, creatinine clearance rate reduced significantly than pentoxifylline group and glycyrrhizin diamine group, Durea/Purea increased more significantly （ F = 7. 824,7.911, P 〈 0.05 ） , pentoxifylline group urea nitrogen and creatinine clearance rate decreased more significantly compared to .glycyrrhizinate diamine group, but the difference were not significant （ P 〉 0.05 ） ; compared with the model group, TNF-α, MCP-1 increased significantly com- pared with pentoxifylline group and glycyrrhizin diamine group （ P 〈 0.05 ） , cyclohexanone theobromine group＇s MCP-1 and TNF-α were lower than Glycyrrhizin acid group, the difference were not significant （ P 〉 0.05）. Conclusion 4.25% glucose peritoneal dialysis solution enables impair rat peritoneal function, glycyrrhizin acid can reduce peritoneal injury, reduce deterioration of peritoneal function.