摘要
蛋白芯片可对生物样本中的靶标分子进行快速筛选,具有高灵敏度和通量化等特点,在转化医学研究中具有巨大的发展潜力。但如何实现大量不同蛋白的高效体内表达和纯化一直是难以解决的瓶颈问题。无细胞表达蛋白芯片技术克服以上不足,提供了一种经济高效的蛋白芯片制备途径。简要综述近年来无细胞表达蛋白芯片技术和在蛋白组学研究中的应用,并就无细胞表达蛋白芯片的发展趋势进行了展望。
Protein microarrays provide a tool for functional proteomics analysis in high-throughput format, and have demonstrated their roles in translational research. However, a major challenge for this technology is the expression and purification of hundreds of proteins using in vivo systems, which is labor intensive and expensive. Cell-free protein microarrays overcome these disadvantages and offer a rapid and cost-effective approach for the generation of protein microarrays. This article gives an overview of the application of cell-free protein microarrays in proteomics and translational research, and the perspective of future cell-free protein microarrays is discussed.
作者
刘磊
代佳宇
王红叶
张苹
杨璐
段虎
于晓波
LIU Lei DAI Jiayu WANG Hongye ZHANG Ping YANG Lu DUAN Hu YU Xiaobo(State Key Laboratory ofProteomics, Beijing Proteorne Research Center, Beijing Institute of Radiation Medicine, National Center for Protein Sciences (The PHOENIX Center, Beijing), Beijing 102206 The General Hospital of the People's Liberation Army, Beijing 100853, China)
出处
《生命的化学》
CAS
CSCD
2017年第1期9-15,共7页
Chemistry of Life
基金
国家自然科学基金面上项目(81673040)
北京市留学人员科技项目择优重点项目
广东省公益研究与能力建设专项资金(2016A020215004)
关键词
蛋白质芯片
无细胞表达
蛋白质组学
转化医学
protein microarray
Cell-free protein expression
proteomics
translational research