无细胞表达蛋白芯片研究进展

Recent Progress of Cell-free protein microarrays

蛋白芯片可对生物样本中的靶标分子进行快速筛选,具有高灵敏度和通量化等特点,在转化医学研究中具有巨大的发展潜力。但如何实现大量不同蛋白的高效体内表达和纯化一直是难以解决的瓶颈问题。无细胞表达蛋白芯片技术克服以上不足,提供了一种经济高效的蛋白芯片制备途径。简要综述近年来无细胞表达蛋白芯片技术和在蛋白组学研究中的应用,并就无细胞表达蛋白芯片的发展趋势进行了展望。

Protein microarrays provide a tool for functional proteomics analysis in high-throughput format, and have demonstrated their roles in translational research. However, a major challenge for this technology is the expression and purification of hundreds of proteins using in vivo systems, which is labor intensive and expensive. Cell-free protein microarrays overcome these disadvantages and offer a rapid and cost-effective approach for the generation of protein microarrays. This article gives an overview of the application of cell-free protein microarrays in proteomics and translational research, and the perspective of future cell-free protein microarrays is discussed.