四溴双酚A对环青春期雌性大鼠的毒性研究

Toxicity study of tetrabromobisphenol A on peripubertal female rats

目的初步探讨四溴双酚A对环青春期雌性大鼠的内分泌干扰作用。方法将19日龄雌性大鼠随机分为4组:对照组和低、中、高剂量组,每组15只,四溴双酚A掺入饲料给予,染毒剂量分别为0、2、6和18 mg/kg BW,连续暴露21 d,试验期间观察并记录大鼠阴道开口时间和动情周期,试验结束后测定各项血液学、临床生化指标以及激素水平,进行脏器称重以及组织病理学检查。结果受试物各剂量组大鼠体质量、阴道开口时间和开口时体质量、动情周期,以及激素水平与对照组相比,差异无统计学意义(P>0.05);各剂量组大鼠肝脏的脏体系数,以及高剂量组甲状腺的脏体系数和低、高剂量组甲状腺的脏脑系数与对照组相比明显降低,差异有统计学意义(P<0.05);血常规检测结果显示中剂量组大鼠血小板计数和血小板压积与对照组相比明显降低,差异有统计学意义(P<0.05);血生化检测结果显示中、高剂量组大鼠胆固醇和血糖含量,以及各剂量组高密度脂蛋白含量与对照组相比明显降低,差异有统计学意义(P<0.05);卵巢、子宫、肾脏、肝脏和甲状腺病理指标未显示有受试物引起的异常改变。结论四溴双酚A对环青春期雌性大鼠肝脏、甲状腺和血糖有影响。

Objective The study aimed to explore the toxicity of tetrabromobisphenol A on peripubertal female rats. Methods Female rats aged 19-day were dietary exposed to tetrabromobisphenol A for 21 days with four dosages（ 0,2,6,18 mg / kg BW）.There were 15 rats in each dosages group. During the experiment,vaginal opening and estrous cyclicity of rats were observed and recorded. Blood samples were collected for blood routine,biochemical indexes and hormone levels. Organ weighing and histopathologic examinations were performed in end point. Results The weight gain,the age of vaginal opening and weight,estrous cyclicity and hormone levels of rats in all the treated groups were similar to those in the non-treated group with no statistically significant difference. Liver / body weight ratio of all the treated groups,thyroid / body weight ratio of the high dosage group and thyroid / brain ratio of the low and high dosage group were significantly decreased. Blood routine showed that platelet count and plateletcrit were significantly decreased in the middle dosage group. Clinical biochemical parameters showed that the total cholesterol and glucose were significantly decreased in the middle and the high dosage group. The level of high density lipoprotein was significantly decreased in all the treated groups. No pathological changes in ovaries,uterus,livers,kidneys and thyroids were observed under microscope. Conclusion Tetrabromobisphenol A etrabromobisprenol may effect on liver,thyroid and glucose metabolism on peripubertal female rats.