利用鳍条组织进行流式细胞实验的可行性研究

Feasibility of flow cytometry experiments based on fin

流式细胞术能快速检测生物细胞的DNA含量和细胞周期,在鱼类遗传育种方面已得到广泛运用。目前的研究多基于鱼类血液和生殖细胞开展实验,存在周期性和操作难度的限制。该研究利用草鱼(Ctenopharyngodon idellus)不同组织进行流式分析,在鳍条组织中得到了明显的峰图,并在4种鱼类的重复实验中得到了稳定结果,且不同鱼类的鳍条组织均存在细胞周期,大部分细胞停留在G_0/G_1期。利用斑马鱼(Danio rerio)作为内标测定出团头鲂(Megalobrama amblycephala)鳍条组织的相对DNA含量(C值)为(1.25±0.07)pg,与相关研究结果基本吻合,认为利用鳍条组织作为血液和生殖细胞的替代材料进行流式细胞实验是科学可行的。

Flow cytometry, which is widely used in fish breeding, can detect cell cycle and DNA content of living organisms rapidly. However, the current studiesmainly focus on experiments based on fish blood and germcells, which have limitation in periodicity and operation. We used different tissues of Ctenopharyngodon idellus by flow cytometry to obtain a desired and stable histogram showing DNA content of the fin tissues from four different fishes. All fin tissues exhibited cell cycle phenomenon, and low ratio appeared at time phase of G0/G1. Besides, Danio rerio was used as internal calibration to detect the DNA content （C value） of Megalobrama amblycephala （1.25±0.07）pg, and the result has no significant difference with the results of other studies. Therefore, it is concluded that use of fin as alternative for blood and germcells for flow cytometry research is scientific and feasible.