去白细胞悬浮红细胞中残留白细胞定量检测新方法的建立

Establishment of novel methods to count the residual leukocytes in red blood cells in additive solution with reduced leukocytes

目的建立一种定量检测去白细胞悬浮红细胞中残留白细胞的新方法。方法使用ADAM-r白细胞计数系统对100份去白细胞悬浮红细胞中白细胞残留量进行检测,同时用流式细胞仪对上述100份标本进行平行检测;并选用3个浓度白细胞残留量的去白细胞悬浮红细胞对ADAM-r白细胞计数系统白细胞残留量检测进行重复性试验。结果 ADAM-r白细胞计数系统和流式细胞仪2种方法检测去白细胞悬浮红细胞中残留白细胞数的相关系数r=0.921,P<0.001,2种方法检测结果具有统计学关联;重复性试验中3个浓度白细胞残留量的去白细胞悬浮红细胞白细胞残留量检测CV值分别为19.1、4.1、3.8。结论可以使用ADAM-r白细胞计数系统定量检测去白细胞悬浮红细胞中的残留白细胞。

Objective To establish novel methods of quantitative detection for residual leukocytes in red blood cells in additive solution with reduced leukocytes. Methods ADAM-r system was used to count residual white blood cells in 100 samples of red blood cells in additive solution with reduced leukocytes. At the same time,flow cytometry was used on the 100 samples for detection. Three concentrations of leukocyte residues in red blood cells in additive solution with reduced leukocytes were chosen using ADAM-r system to test repeatability of WBC count. Results The correlation coefficient of the two methods,ADAM-r leucocyte counting system and flow cytometry instrument was r = 0. 921（ P〈0. 001） which suggested that the results of these two methods were statistically associated. The variable coefficient of the three concentration＇s repeatability test were 19. 1,4. 1,and 3. 8,respectively. Conclusion ADAM-r white blood cell count system can be used for quantitative detection of residual leukocytes in red blood cells in additive solution with reduced leukocytes.