摘要
目的探讨体外冲击波(extracorporeal shock wave,ESW)对破骨细胞形成及骨吸收作用的影响。方法骨髓源巨噬细胞(bone marrow-derived macrophages,BMMs)经巨噬细胞集落刺激因子(macrophage colony-stimulating factor,M-CSF)与核因子κB受体活化因子配体(receptor activator of nuclear factorκB ligand,RANKL)诱导培养4天,同时给予不同能量的ESW干预,4天后进行抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphates,TRAP)染色鉴定破骨细胞并进行破骨细胞计数,逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测破骨细胞分化相关基因NFATc1和c-Fos的表达,骨板吸收试验检测破骨细胞的骨吸收活性。结果 BMMs经诱导4天后出现大量的破骨细胞,ESW干预后破骨细胞的数量显著减少,且吸收面积缩小,破骨细胞分化相关基因NFATc1和c-Fos的表达均显著下降,高能量ESW干预组的抑制效果更明显(P<0.05)。结论适当能量的ESW能够通过抑制NFATc1和c-Fos基因表达而遏制体外培养的破骨细胞的分化;ESW能够明显抑制成熟破骨细胞的骨吸收作用。
Objective To study the effect of extracorporeal shock wave （ESW） on osteoclast formation and resorption of bone. Method Bone marrow-derived macrophages （BMMs） were induced with macrophage colony-stimulating factor （M-CSF） and receptor activator of nuclear factor κB ligand （RANKL） for 4 days, meanwhile, interfered with ESW of different energy. After 4 days, tartrate-resistant acid phosphates （TRAP） staining was used to identify the osteoclasts and count osteoclast cells, so as to analyze the effect of ESW on osteoclast formation; Using reverse transcription-polymerase chain reaction （RT-PCR） technology to analyze NFATc1 and c-Fos gene expression of different groups. The resorption pits on the surface of bovine bone slice reflecting the activity of osteoclasts. Result BMMs were induced into TRAP positive osteoclasts with M-CSF and RANKL for 4 days. The number of osteoclasts was reduced and the expression level of osteoclast relative gene NFATc1 and c-Fos was down regulated by ESW; ESW ameliorated the bone destruction on the surface of bovine bone slice （P 〈 0.05）. Conclusion A certain energy intensity of ESW could suppresses osteoclast differentiation by inhibiting NFATc1 and c-Fos gene expression; ESW decrease bone resorption area.
出处
《中国医学前沿杂志（电子版）》
2017年第2期20-24,共5页
Chinese Journal of the Frontiers of Medical Science(Electronic Version)
基金
国家自然科学基金项目(11405185)
