电针“委中”对大鼠腰多裂肌损伤后LIF、IL-17表达的影响

Effects of electroacupuncture “Weizhong” (BL 40) on the expression of LIF and IL-17 in rats with multifidus muscle injury

目的观察电针"委中"对大鼠腰多裂肌损伤后组织形态学变化以及对骨骼肌肌肉因子白血病抑制因子(leukemia inhibitory factor,LIF)、骨骼肌损伤前炎症因子白介素17(interleukin,IL-17)表达含量的影响。方法将90只雄性SD大鼠随机分为空白组、模型组、模型对照组、电针委中组、电针肾俞组,每组18只。每组再随机分为1天、3天、7天三个时间点。麻醉后于双侧L4-5多裂肌注射0.5%布比卡因溶液复制多裂肌损伤模型,通过光镜观察分析造模后多裂肌形态结构的变化。通过ELISA和免疫组化观察1天、3天、7天多裂肌LIF、IL-17表达含量的动态时相变化。结果造模后,与空白组比较,模型组不同时间点的LIF及IL-17表达含量均明显增加(P<0.01);与模型组比较,电针委中和电针肾俞组的IL-17表达含量有所降低(P<0.01),而LIF的表达含量有所增加(P<0.01);与电针肾俞组比较,电针委中组在1天后下调IL-17的表达含量更明显(P<0.05),在3天、7天后上调LIF的作用更显著(P<0.05)。结论电针委中可促进IL-17的表达下调,减轻炎症反应的程度,同时可增加LIF的表达含量,促进骨骼肌中葡萄糖的摄取,利于骨骼肌修复。

Objective To observe the effect of electroacupuncture(EA) stimulate at'Weizhong'(BL 40) on the expression of leukaemia inhibi-tory factor(LIF) and interleukin-17(IL-17) in rats with multifidus muscle injury. Methods A total of 90 rats were randomly divided into blank group,model control group,model group,EA'Weizhong'(BL 40) group and a EA'Shenshu'(BL 23) group,18 rats in each group. Each group was randomly divided into a 1-day subgroup,a 3-day subgroup and a 7-day subgroup again,6 rats in each subgroup. Both sides of multifidus muscle(L4 and L5) were injected with 0. 5%bupivacaine. The morphological and ultrastructural changes of multifidus muscle were observed and analyzed with light microscope at 1st,3rd,7th day after model establishment. The expression of leukaemia inhibi-tory factor(LIF) and interleukin-17(IL-17) was measured by immunohistochemical method and elisa method. Results After modeling,compared with the blank group,the expression of LIF and IL-17 in multifidus muscle of model group was significantly increased(P < 0. 01); Compared with the model group,theexpression of the LIF were significantly increased(P < 0. 01) and the expression of IL-17 in multifidus muscle was significantly decreased(P < 0. 01) in the EA 'Weizhong'group and EA 'Shenshu'group. Compared with EA 'Shenshu'group,the expression of the IL-17 in multifidus muscle was significantly decreased at 1th day; the expression of LIF was significantly increased at 3th day and 7th day. Conclusion EA 'Weizhong'(BL 40) can increase the expression of LIF and decrease the expression of IL-17,and can reduce inflammatory reaction and promote skeletal muscle glucose uptake,so as to be beneficial to skeletal muscle repair.