乳腺癌超声征象与P120ctn、Ki67表达水平的相关性

Correlation between ultrasonic sign and the expression level of Ki67 and P120ctn in patients with breast cancer

目的探讨连环蛋白P120ctn和核增殖相关蛋白Ki67表达对乳腺癌超声征象的影响。方法选择乳腺癌患者组织标本124例,对相关生物样本的分析按照乳腺癌诊治规范进行。分析所有样本的超声征象,如肿块边缘、大小、形态、钙化区域、淋巴结转移、血流显像分级等;采用免疫组织化学法检测组织样本中P120ctn和Ki67的表达水平;分析其与乳腺癌超声征象的相关性。结果 Ki67在乳腺癌组织中呈现阳性表达,P120ctn在乳腺癌组织中呈现阴性表达;在不同肿块直径和边缘存在毛刺的组织中,P120ctn和Ki67的表达水平差异均无统计学意义(P>0.05),而在不同肿块形态、不同分布的微小钙化点、不同血流显像分级和发生淋巴结转移与否的组织中,P120ctn和Ki67的表达差异均有统计学意义(P<0.05)。P120ctn蛋白和Ki67蛋白表达与超声征象中的肿块形态、微小钙化点分布、血流显像分级和淋巴结转移特点存在联系。Ki67和P120ctn蛋白表达之间呈正相关(P<0.05)。结论超声征象与P120ctn蛋白和Ki67蛋白表达之间存在一定的联系,可以作为无创性预测乳腺癌患者P120ctn蛋白和Ki67蛋白表达的方式,为乳腺癌恶变程度和预后的判断提供更多的依据。

Objective To investigate the effect of P120ctn and Ki67 expression on breast cancer ultrasonography. Methods The breast cancer tissues of 124 patients with breast cancer who were examined and treated in our hospital. Analysis of the relevant biological samples was in accordance with the standard of diagnosis and treatment of breast cancer. The ultrasonic features of all the samples were analyzed, such as the edge, size, shape, calcification, lymph node metastasis, blood flow imaging and so on. The expression level of Ki67 and P120ctn in tissue samples was detected by immunohistochemical method, and their correlation with the ultrasonic features of breast cancer were analyzed. Results The expression of Ki67 in breast cancer tissues was positive, and the expression of P120ctn in breast cancer tissues was negative. There was no significant difference in the expression levels of P120ctn and Ki67 between the tissues with different diameters and edges（P〉0.05）. However, the expression of P120ctn and Ki67 were significantly different in the tissues with different shape, different distribution of microcalcifications, different blood flow imaging grade and lymph node metastasis （P〈0.05）. The expression of P120ctn protein and Ki67 protein was related to the shape of the tumor, the distribution of micro calcification, the grade of blood flow imaging and the characteristics of lymph node metastasis. The expression of P120ctn and Ki67 were negatively correlated（P〈0.05）. Conclusion The results showed that there was a certain relationship between ultrasonic signs and the expression of P120ctn protein and Ki67 protein. Ultrasonographic signs can be used as noninvasive prediction of the expression of P120ctn and Ki67 protein in patients with breast cancer to provide more evidence for the degree of malignancy and prognosis of breast cancer.