激素替代-冻融胚胎移植周期子宫内膜的最佳容受时间研究

Optimal time of endometrial receptivity in hormone replacement therapy-frozen embryo transfer cycles

目的探讨激素替代-冻融胚胎移植(HRT-FET)周期子宫内膜的最佳容受时间。方法根据子宫内膜暴露于黄体酮天数的不同(3、4、5、6和7 d),将52例行HRT-FET的患者依次定义为P3组、P4组、P5组、P6组及P7组,分别观察各组胞饮突形态及覆盖面积,并检测整合素αvβ3、同源框基因A10(HOXA10)及LIF3种蛋白的相对表达量。结果多数患者在黄体酮补充6 d时,其子宫内膜胞饮突发育程度与αvβ3、HOXA10及LIF 3种蛋白表达水平一致性达到最高。结论将胞饮突发育程度检测作为冻融胚胎移植时间评估的参考依据是可行,于黄体酮补充3 d时对胚胎进行移植,可能是实现子宫内膜与胚胎同步化的理想选择。

Objective To investigate the optimal time of endometrial receptivity in hormone replacement therapy-frozen embryo transfer （HRT-FET） cycles. Methods According to days of exposure to progesterone, 52 cases undergoing HRT-FET were divided into P3 group, P4 group, P5 group, P6 group and P7 group. Pinopode morphology, coverage area, integrin alpha v beta 3 （αvβ3）, homeobox gene A10 （HOXA10） and leukemia inhibitory factor （LIF） were detected and compared among the groups. Results Most of the cases had the highest expression of αvβ3, HOXA10 and LIF after 6-day exposure to progesterone. Conclusions It is feasible to evaluate the degree of sporadic development of embryos as embryo transfer time. The transplanta-tion of the embryo on the 3rd day of progesterone supplementation may be an ideal choice for the synchro-nization of endometrium and embryo.