内皮克隆形成细胞条件培养基对人真皮成纤维细胞生物学作用的影响

Effect of endothelial colony-forming cells conditioned medium on biological function of human dermal fibroblasts

目的:探讨内皮克隆形成细胞条件培养基(ECFCs-CM)对人真皮成纤维细胞(HDFs)生物学作用的影响。方法:从人脐带血中分离内皮克隆形成细胞(ECFCs)并鉴定;采用抗体芯片检测ECFCs-CM中细胞因子表达谱;将ECFCs-CM作用于HDFs,EBM-2作为对照,采用CCK-8法、细胞划痕实验及流式细胞术分别检测细胞的增殖、迁移及凋亡情况。结果:原代培养细胞符合ECFCs的特征;ECFCs-CM相对高表达PDGF-BB、EGF等细胞因子;实验组HDFs增殖能力和迁移能力均高于对照组,且在血清饥饿环境下实验组HDFs凋亡率低于对照组。结论:ECFCs-CM可促进HDFs增殖和迁移,并可抑制其在血清饥饿环境下的凋亡。

Objective To examine the effect of endothelial colony-forming cells conditioned medium（ECFCs- CM） on biological function of human dermal fibroblasts （HDFs）. Methods Human cord blood derived-ECFCs were isolated and identified based on the previous studies. The cytokines in ECFCs-CM were detected using a cytokines antibody array. HDFs were cultured with ECFCs-CM, using serum free EBM-2 as control. The proliferation of HDFs was examined by Cell Counting Kit-8 （CCK-8） and the migration was assessed by scratch test assay. The apoptosis of HDFs was detected by flow eytometry. Results The cells isolated from human cord blood demonstrated typical characteristics of ECFCs. The cytokines antibody array indicated that ECFCs-CM contained large amounts of secreted cytokines such as PDGF-BBand EGF. Compared with the control group, the HDFs cultured with ECFCs-CM showed improved proliferation and migration ability. The number of apoptotic cells was smaller than that of the control group under the environment of serum starvation. Conclusion ECFCs-CM can promote the proliferation and migration of HDFs and inhibit the apoptosis of HDFs under the environment of serum starvation.