乌榄叶水提取物对大鼠离体心脏缺血再灌注损伤的保护作用

Protective effects of extracts from leaves of Canarium pimela Leenh on myocardial ischemia-reperfusion injury in rats

目的:明确乌榄叶水提取物对大鼠离体心脏缺血再灌注损伤的保护作用。方法:采用Langendorff离体心脏灌流术,结扎冠脉左前降支35 min,分别复灌10 min和50 min制备不同程度缺血再灌注损伤模型。用乌榄叶水提物制备灌流液对离体心脏进行灌注并观察:(1)乌榄叶水提物预处理对正常离体心脏功能的影响;(2)乌榄叶水提物预处理10 min后制备缺血再灌注模型,观察:(1)左心室功能及冠脉流量;(2)室颤或室速发生率;(3)冠脉流出液中肌酸激酶及乳酸脱氢酶活性;(4)缺血再灌注损伤的病理变化。结果:乌榄叶水提取物可有效提高正常左心室功能,且可有效降低不同程度再灌注损伤引起的左室LVEDP及+dp/dtmax的变化,提高心脏顺应性,降低室颤或室速的发生率,并抑制冠脉流出液中肌酸激酶及乳酸脱氢酶活性以及缺血再灌注引起的心肌病变。结论:乌榄叶水提取物对大鼠离体心脏缺血再灌注损伤具有保护作用。

Objective To study the effect of liquid extracts from leaves of Canarium pimela Leenh on myo- cardial isehemia-reperfusion injury in rats. Methods The langendorff isolated perfused heart system was applied in this study. Ligating of the left descending anterior for 35 rain, followed with 100 min or 50 min reperfusing to set up the cardiac ischemia-reperfusion injury model （I/R）. After perfusing the effective pharmacological extracts of leaves of Canarium pimela Leenh （CPL） to the isolated heart, we monitored the cardiac parameters of left ventricu- lar systolic pressure （LVSP） , left ventricular end- diastolic pressure （LVEDP） and left ventrieular maximal rise/ fall of left ventricular pressure （dp/dtmax） in the following assays with or without 10 min CPL pretreatment. 1. the cardiac parameters, 2. the cardiac parameters in 35 rain ischemia, followed with 10 min or 50 min reperfusion, 3. the incidence of ventricular fibrillation or ventricular taehycardia after 10 min reperfusion, 4. the activity of creatine kinase （CK） and lactate dehydrogenase （LDH） in the coronary effluent after 10 min reperfusion, 5. pathological analysis in the I/R, CPL and VER group after reperfusion. Results CPL pretreatment improved functions of normal left heart. Furthermore, it significantly reduced LVEDP and ± dp/dt the incidence of ventricular fibrillation or ventricular tachycardia, as well as the activities of CK and LDH in coronary effluent induced by isehemia-reperfusion compared with the I/R model. Moreover, CPL pretreatment markedly alleivated the pathological changes of ischemia- reperfusion. Conclusions The liquid extracts of leaves of Canarium pimela Leenh can effectively relieve the myocardial isehemia-reperfusion injury in rats.