肺腺癌患者CD4^+CD29^+T细胞含量与转移及放疗的关系

Correlation between CD4^+CD29^+T cells and metastasis and radiotherapy for patients with pulmonary adenocarcinoma

目的:观察肺腺癌患者CD4^+CD29^+T细胞含量与转移及放疗的关系。方法:收集71例肺腺癌患者为观察对象,并以非肺腺癌、慢性阻塞性肺疾病(COPD)患者及健康志愿者作为对照,比较血CD4^+CD29^+T细胞及其胞内肿瘤坏死因子α及白介素1(IL-1)的含量差异;比较转移与未转移、放疗前后肺腺癌患者上述指标及整合素β1、血管内皮生长因子(VEGF)含量的差异,并分析其相关性。结果:肺腺癌及非肺腺癌患者均显著高于COPD及健康组,且肺腺癌组显著高于非肺腺癌组(P<0.05);肺腺癌转移患者的整合素β1、VEGF及CD4^+CD29^+T细胞、TNF-α及IL-1均显著高于未转移组(P<0.05);放疗后肺腺癌患者CD4^+CD29^+T细胞、TNF-α及IL-1均显著低于放疗前(P<0.05);CD4^+CD29^+T细胞、TNF-α及IL-1三者均与整合素β1及VEGF呈显著正相关性。结论:CD4^+CD29^+T细胞及其细胞因子在肺腺癌患者外周血表达增加,且与转移及放疗预后密切相关,具有重要临床意义。

Objective To observe the correlation between CD44^＋CD29^＋ T cells and metastasis and radiotherapy for patients with pulmonary adenocarcinoma. Method Seventy-one patients with lung adenocarcinoma, 93 patients with lung adenocarcinoma, 76 cases of chronic obstructive pulmonary disease （COPD） , 63 cases of healthy volunteers were enrolled. Frequencies of blood CD4^＋CD29^＋T ceils and their intracellular necrosis factor alpha （TNF-α） and interleukin 1 （IL-1） were compared. Compare TNF-α, IL-1, integrin beta 1 and vascular endothelial growth factor （VEGF） levels in the patients with transferred pulmonary adenocarcinoma or with non- transferred pulmonary adenocarcinoma and their changes with the treatment of radiotherapy. Results the patients with lung adenocarcinoma and non lung adenocarcinoma were significantly higher than that of COPD and health group, and patients with lung adenocarcinoma is significantly higher than patients with non lung adenocarcinoma （P 〈 0.05） ; Integrin beta 1, VEGF and CD4^＋CD29^＋ T cells, TNF-α and IL-1 level in patients with lung adeno- carcinoma metastasis were significantly higher than non-transferred group （P 〈 0.05） ; After radiotherapy, CD44^＋CD29^＋ T cells, TNF-α and IL-1 in patients with lung adenocarcinoma were significantly lower than before （P 〈 0.05 ） ; CD44^＋CD29^＋ T cells, TNF alpha and IL-1 with integrin beta 1 and VEGF had significantly positive correlations. Conclusion CD44^＋CD29^＋ T cells and cytokines increase significantly in the blood of patients with lung adenocarci- noma, and are related to the prognosis of metastasis and radiation therapy, which has important clinical significance.