NEP1-40对缺氧缺血性脑病新生大鼠的Wnt信号通路胞增殖的调控作用

Role of NEP1-40 in regulation of Wnt signaling pathway and regeneration of neural cells in neonatal rats with hypoxic ischemic encephalopathy

目的探讨Nogo-A受体拮抗剂NEP1-40对Wnt信号通路和神经细胞增殖的调控作用。方法 40只大鼠被均分为HIBD(缺氧缺血性脑损伤)组和HIBD+NEP1-40组,采用PCR定量、Western blot分析、细胞增殖的免疫组化试验、8-异前列腺素评估等检测分析缺氧缺血性脑病新生大鼠的修复过程中Wnt信号通路中NgR的转录因子调控与神经细胞增殖。结果NEP1-40处理后,c Jun和c-Myc的表达在蛋白水平上调,基因表达水平上调,Ki-67增加,8-异前列腺素无显著变化。结论通过抑制NgR后发现,c-Jun和c-Myc是Wnt通路的主要转录因子,同时脑室下区神经细胞的增殖增加。

Objective To explore the role of Nogo-A receptor antagonist NEP1-40 in regulating regeneration of neural cells and related Wnt signaling pathway in neonatal rats with hypoxic ischemic encephalopathy（HIBD）.Methods A total of 40 HIBD rats were divided into HIBD group and HIBD ＋ NEP1-40 group,20 rats in each group.PCR Test,Western Blot Analysis,IHC test for cell proliferation and 8-isoprostane detection were used to evaluate regulation of NgR transcription factors in Wnt signaling pathway and proliferation of neural cells.Results The expressions of c-Jun and cMyc,at the protein level,were up-regulated after treatment with Nogo-A receptor antagonist NEP1-40 for 7 days,and the same change was observed at gene expression and Ki-67.There was no significant change of 8-isoprostane.Conclusion The c-Jun and c-Myc are the main transcription factors in Wnt signaling pathway by inhibition of NgR,and meanwhile the proliferation of nerve cells in subventricular zone increase.