摘要
目的:探讨白介素17A(IL-17A)对白色念珠菌Candida albicans(C.a)感染的人口腔黏膜上皮细胞(OMECs)β-防御素-2(hBD-2)表达的影响。方法:将OMECs分为空白对照组、单独重组人IL-17A(rhIL-17A)处理组、单独C.a处理组(模型组)及C.a与rhIL-17A共同处理组(实验组)。按菌∶细胞=1∶10建立C.a感染的OMECs模型。分别将单独rhIL-17A处理组和实验组的OMECs按所加入的rhIL-17A浓度分为4个亚组:1μg/L组、10μg/L组、100μg/L组及1 000μg/L组,共培养48h。收集不同时间点(0h、12h、24h、48h)的细胞培养上清液,计算C.a黏附率;分别采用荧光定量PCR(FQ-PCR)和酶联免疫吸附试验(ELISA)方法检测hBD-2mRNA相对表达量及蛋白水平。结果:与模型组比较,实验组C.a黏附率有所降低,但差异无统计学意义(P>0.05)。不同浓度的rhIL-17A均能诱导OMECs表达hBD-2,且100μg/L组hBD-2mRNA相对表达量及蛋白水平升高最为显著(P<0.01)。采用100μg/L rhIL-17A刺激OMECs 12h、24h、48h后,hBD-2mRNA及蛋白水平均升高,且48h时hBD-2mRNA及蛋白水平显著高于0h和12h(P<0.05或P<0.01)。结论:IL-17A能诱导OMECs表达hBD-2,但对口腔黏膜上皮抗C.a黏附无明显作用。
Objective：To investigate the effect of interleukin 17A （IL-17A） on the expression of ^-defensin-2 （hBD^2） and the adherence of C. albicans to human oral mucosal epithelial cells （OMECs）. Methods： OMECs was divided into 4 groups： a control group, an only recombinant human IL-17A （rhIL-17A） intervention group, a model group in which the ceils were infected with C. albicans （1 ：10 ratio）, and an experimental group in which the cells were infected with C. albicans and treated with different concentrations （1, 10, 100 and 1,000 μg/L） of rhIL-17A. The mRNA and protein levels of hBD-2 were detected by fluorescent quantitative PCR （FQ-PCR） and enzyme-linked immuno sorbent assay （ELISA）, respectively. Results：C. albicans adherence rate in the experimental group was a little lower than that in the model group （ P 〉0.05）. Different concentrations of rhIL-17A could up-regulate hBD-2 expression in OMECs, and 100 μg/L was the highest （ P 〈0. 01）. Treated with 100 μg/L rhIL-17A for 12 h, 24 h and 48 h lead to markedly increased hBD-2 mRNA and protein levels in OMECs, but 48 h was the highest （ P 〈0.05 or P 〈0.01）. Conclusion：IL-17A could induce the hBD-2 expression in OMECs, whereas had no effect on the adherence of C. albicans to OMECs.
出处
《广西医科大学学报》
CAS
2017年第4期510-513,共4页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81260167)
