马钱子碱固体脂质纳米粒在小鼠体内的组织分布

Tissue distribution of brucine-loaded solid lipid nanoparticles in mice in vivo

目的研究马钱子碱固体脂质纳米粒在小鼠体内的组织分布。方法制备马钱子碱固体脂质纳米粒,以异硫氰酸荧光素(FITC)标记。小鼠静脉注射纳米粒混悬液,HPLC法测定各组织(心、肝、脾、肺、肾、骨)中马钱子碱含有量,分析纳米粒体内组织分布,再采用荧光内窥式激光共聚焦显微镜作进一步检测。结果马钱子碱在小鼠肝脏中的相对摄取率(Re)最高(1.64)。纳米粒在各组织中的靶向效率(Te)均大于1,对肝脏的选择性明显强于马钱子碱溶液。随着时间延长,FITC标记的纳米粒由细胞外逐渐分布到细胞内,数量亦逐渐增加,并呈正相关性。结论马钱子碱制成固体脂质纳米粒后,可提高其在小鼠肝组织中的分布,具有较明显的肝靶向性。

AIM To investigate the tissue distribution of brucine-loaded solid lipid nanoparticles in mice in vivo. METHODS Mice were intravenously injected with suspension of prepared brucine-loaded solid lipid nanoparticles and marked by fluorescein isothiocyanate （FITC）. The in vivo tissue distribution of nanoparticles was analyzed by having the brucine contents in various tissues （heart, liver, spleen, lung, kidney and bone） determined by HPLC, after which fluorescence confocal laser endomicroscopy was used for further detection. RESULTS Brucine had its the highest （1.64） relative intake efficiency （Re） in mice liver, and the nanoparticles shared all over one value of targeting efficiencies （Te） in various tissues, manifesting a much stronger selectivity to liver than that of brucine solution. With the extension of time, the FITC-marked nanoparticles displayed a rich extracellular to intracellular distribution indicating a positive correlation. CONCLUSION Brucine＇ s increased distribution in the liver tissue of mice due to its solid lipid nanoparticle form shows obvious for liver targeting.