青钱柳质量标准的研究

Quality standard for Cyclocarya paliurus

目的建立青钱柳Cyclocarya paliurus(Batal.)ljinsk.的质量标准。方法测定12批样品水分、灰分、浸出物含有量,TLC法定性鉴别槲皮素和山柰酚,HPLC法定量测定其含有量,苯酚-浓硫酸显色法测定多糖含有量。结果水分、总灰分、酸不溶性灰分、水浸出物、醇浸出物平均含有量分别为11.05%、5.81%、1.70%、11.25%、10.16%。TLC斑点清晰,专属性强。槲皮素、山柰酚分别在0.004 953~0.022 29、0.005 748~0.028 74 mg/mL范围内呈良好的线性关系(r=0.999 9),平均加样回收率分别为97.1%(RSD=2.59%)、97.9%(RSD=2.86%);多糖在0.021 22~0.095 58 mg/mL范围内线性关系良好(r=0.999 8),平均加样回收率97.2%(RSD=2.42%)。在各批样品中,3种成分含有量均有明显差异。结论青钱柳中水分、总灰分、酸不溶性灰分、水浸出物、醇浸出物含有量分别不得超过13.0%、7.0%、2.0%、13.5%、12.0%,而槲皮素、山柰酚、多糖含有量以干燥品计,分别不得低于0.040%、0.070%、0.60%。

AIM To establish the quality standard for Cyclocarya paliurus （ Batal. ） ljinsk.. METHODS The contents of water, ash and extract in twelve batches of samples were determined. TLC and HPLC were adopted in the qualitative identification and quantitative determination of quercetin and kaempferol, respectively, and phenol-sulfuric acid method was used for the polysaccharide content assessment. RESULTS The average contents of water, total ash, acid-insoluble ash, water-soluble extract and alcohol-soluble extract were 11.05% , 5.81% , 1.70% , 11.25% and 10. 16% , respectively. The clear TLC spots demonstrated their strong specificity. Quercetin and kaempferol showed good linear relationships within the ranges of 0. 004 953 - 0. 022 29 mg/mL and 0. 005 748 - 0. 028 74 mg/mL （ r = 0. 999 9）, whose average recoveries were 97. 1% （ RSD = 2. 59% ） and 97.9% （ RSD = 2. 86% ）, respectively. Polysaccharide showed a good linear relationship within the range of 0. 021 22 -0. 095 58 mg/mL, whose average recovery was 97.2% （ RSD = 2. 42% ）. The contents of three constituents in various batches of samples showed obvious differences. CONCLUSION In C. paliurus, the contents of water, total ash, acid-insoluble ash, water-soluble extract and ethanol-soluble extract should not be more than 13.0% , 7.0% , 2. 0% , 13.5% and 12. 0% , while those of quercetin, kaempferol and polysaccharide （calculated by dry product） should not be less than 0. 040% , 0. 070% and 0. 60% , respectively.