摘要
采用传统纯培养方法对采集自新疆阿勒泰地区的15份传统酸乳样品中的乳酸菌进行分离纯化,运用16S rDNA序列分析方法进行属种鉴定。结果表明,分离的36株乳酸菌属于乳杆菌属(Lactobacillus)的5个种或亚种。其中发酵乳杆菌(Lactobacillus fermentum)8株,植物乳杆菌(Lactobacillus plantarum)6株,开菲尔乳杆菌(Lactobacillus kefiri)6株,干酪乳杆菌(Lactobacillus casei)3株,鼠李糖乳杆菌(Lactobacillus rhamnosus)6株,德氏乳杆菌保加利亚亚种(Lactobacillus bulgaricus)3株,瑞士乳杆菌(Lactobacillus helveticus)4株。其中发酵乳杆菌为新疆阿勒泰地区传统酸乳中的优势菌种,占总分离株的22%。
36 lactic acid bacteria were isolated from 15 yogurt samples collected in Altay, Xinjiang. They were identified by sequence analysis of 16 S rDNA. The microtlora in Altay traditional yogurts include 8 strains of Lactobacillus fermentum, 6 strains of Lactobacillus plantarum, 6 strains of Lactobacillus kefiri, 3 strains of Lactobacillus casei, 6 strains of Lactobacillus rhamnosus, 3 strains of Lactobacillus bulgaricus, and 4 strains of Lactobacillus helveticus. It indicated that Lactobacillus fermentum was the dominant bacteria in the traditional yogurts in Altay, Xinjiang.
出处
《食品科技》
CAS
北大核心
2017年第3期22-25,共4页
Food Science and Technology
基金
河北省重大成果转化项目(14047104Z)
关键词
乳酸菌
分离鉴定
16S
RDNA序列分析
lactic acid bacteria
isolation and identification
16S rDNA sequence analysis
