摘要
采用半静水式实验方法,研究Pb^(2+)暴露30 d对中华大蟾蜍(Bufo bufo gargarizans Cantor)生理状态和耳后腺分泌蟾蜍甾烯水平的影响。结果表明,随Pb^(2+)浓度(0.68~5.46 mg·L^(-1))升高,蜕皮和自发活动减少,存活率下降。进一步采用液相-质谱检测技术发现,0.68 mg·L^(-1)Pb^(2+)暴露下,10种蟾蜍甾烯水平出现1.5倍以上变化。5.46 mg·L^(-1)Pb^(2+)暴露下,17种蟾蜍甾烯水平发生下调(>1.5倍),其中bufarenogin水平显著下降(P<0.05),其他物质水平与对照组无显著性差异。这说明Pb^(2+)暴露对蟾蜍耳后腺中蟾蜍甾烯物质具有潜在影响。
The effect of Pb^2+exposure for 30 days on the physiological status of Bufo bufo gargarizans Cantor and on the levels of bufadienolides in toad venom were studied, using the semi-static water design. With the increase of Pb^2+concentration from 0.68 to 5.46 mg·L^-1, the toad's molting and spontaneous activity were remarkably inhibited, and the toad mortality increased. Furthermore, ultra performance liquid chromatography-mass spectrometry was used to monitor the relative levels of bufadienolides in toad venom. We found 10 kinds of bufadienolides with more than 1.5-fold change in 0.68 mg·L^-1Pb^2+exposure group compared with control group. In Pb^2+exposure group(5.46 mg·L^-1), 17 kinds of bufadienolides were down-regulated with more than 1.5-fold change, in which the levels of bufarenogin was decreased significantly(P〈0.05), while others had no significant change. These illustrated that Pb^2+exposure could affect the levels of bufadienolides in toad venom.
出处
《生态毒理学报》
CAS
CSCD
北大核心
2017年第1期212-218,共7页
Asian Journal of Ecotoxicology
基金
江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心资助项目(FJGJS-2015-15,ZDXM-1-14)
江苏省科技厅产学研项目(BY2015008-01)
江苏省六大人才高峰C类(YY-015)
国家自然科学基金(No.81673563,81102762,3901894,81274199)
江苏高校优势学科建设工程资助项目(PAPD)
国家中药标准化和江苏省科技成果转化项目(ZYBZH-C-JS-30,BA2016104)
关键词
铅离子
中华大蟾蜍
蟾蜍甾烯
Pb2+
Bufo bufo gargarizans Cantor
bufadienolides