线叶金雀花对小鼠脑缺血再灌注损伤的保护作用

Protective effect of Rooibos on cerebral ischemia-reperfusion injury in mice

目的观察南非特有植物线叶金雀花对小鼠脑缺血再灌注损伤的保护作用。方法将小鼠随机分为对照组,脑缺血再灌注损伤组,线叶金雀花低剂量组(2.8 g/kg)、中剂量组(5.6 g/kg)、高剂量组(11.2 g/kg)。造模前预防性灌胃给药7 d,每日1次,对照组及脑缺血再灌注组给予等体积的蒸馏水。给药1周后,采用小鼠大脑中动脉栓塞法(MCAO)制备模型,模型制备成功后继续按上述方式灌胃给药7 d。通过2,3,5一氯化三苯基四氮唑(TTC)染色,观察线叶金雀花对脑损伤梗死体积的影响;通过神经功能评分实验、平衡木实验、Morris水迷宫实验、转角实验等,观察线叶金雀花对运动、认知、感觉等功能的影响;通过检测脑组织中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量,观察药物对脑损伤以后氧化还原状态的影响;通过检测脑组织中白介素1β(IL-1β)、肿瘤坏死因子(TNF-α)、细胞间粘附分子-1(ICAM-1)含量,观察药物对脑损伤以后炎症状态的影响。结果与脑缺血再灌注损伤组相比,线叶金雀花组小鼠脑梗死体积显著减少(P<0.05),平衡木得分显著降低(P<0.01),转角向非损伤侧次数显著减少(P<0.01),脑组织TNF-α含量、IL-1β含量与ICAM-1含量显著降低(P<0.01或P<0.05)。结论线叶金雀花对小鼠脑缺血再灌注损伤有一定保护作用,其作用可能与减少脑组织炎症因子有一定关系。

Objective To observe the protective effect of Rooibos（ endemic plant in the South Africa） in mice with cerebral ischemia-reperfusion injury（ IRI）. Methods All mice were divided into control group,IRI group,low-dose Rooibos group（ low-dose group,2. 8 g/kg）,mid-dose Rooibos group（ middose group,5. 6 g/kg） and high-dose Rooibos group（ high-dose group,11. 2 g/kg）. Before modeling,3 medicated groups were orally given Rooibos once a day for 7 d,and control group and IRI group were given isometric distilled water. After one week,the model was established by using cerebral middle cerebral artery occlusion（ MCAO）,and then all groups were continuously given Rooibos or distilled water for 7 d. The influence of Rooibos on cerebral infarction volume was observed after TTC staining.The influence of Rooibos on movement,cognition and sense was observed after neurologic score test,balance beam test,Morris water maze（ MWM） test and corner test. The influence of Rooibos on redox state after cerebral injury was observed after detecting the activities of superoxide dismutase（ SOD） and glutathione peroxidease（ GSH-PX）,and content of malondialdehyde（ MDA）. The influence of Rooibos on inflammatory state after cerebral injury was observed after detecting the brain contents of interleukin-1β（ IL-1β）,tumor necrosis factor-α（ TNF-α） and intercellular adhesion molecule-1（ ICAM-1）.Results The cerebral infarction volume decreased significantly（ P 〈 0. 05）,scores of balance beam test decreased significantly（ P 〈 0. 01）,times from corner to non-injury side decreased significantly（ P 0. 01）,and contents of TNF-α,IL-1β and ICAM-1 decreased significantly（ P〈 0. 01 or P 〈0. 05） in 3Rooibos groups compared with IRI group. Conclusion Rooibos has certain protective effect in mice with cerebral IRI,and the effect may be related to the decrease of inflammation factors in brain tissue.