化浊解毒方对慢性糜烂性胃炎浊毒内蕴证患者蛋白激酶B及肝细胞生长因子受体表达的影响

Influence of Huazhuo Jiedu Fang on Akt and c-Met expressions in patients with chronic erosive gastritis(syndrome of turbidity-toxin accumulating in the interior)

目的观察化浊解毒方治疗慢性糜烂性胃炎(CEG)浊毒内蕴证患者的临床疗效,并从治疗前后血清和胃黏膜组织中蛋白激酶B(Akt)、肝细胞生长因子受体(c-Met)表达水平的变化方面探讨化浊解毒方的作用机制。方法采用随机单盲对照临床试验设计,将80例患者随机分为2组,治疗组予化浊解毒方治疗,对照组予阿拉坦五味丸治疗,观察2组患者治疗前后血清及胃黏膜组织中Akt、c-Met的表达水平。结果治疗后,治疗组血清和胃黏膜组织Akt表达水平较对照组明显降低(P<0.05),治疗组胃黏膜组织c-Met表达水平较对照组明显降低(P<0.05),差异有统计学意义。结论化浊解毒方能显著降低血清及胃黏膜组织中Akt、c-Met的表达水平,其作用机制可能是该方通过调控Akt、c-Met的表达水平及其活性而对CEG产生作用。

Objective To observe the clinical efficacy of Huazhuo Jiedu Fang in patients with chronic erosive gastritis（ CEG,syndrome of turbidity-toxin accumulating in the interior）,and investigate its effect mechanism based on the expression changes of protein kinase B（ Akt） and hepatocyte growth factor receptor（ c-Met） in gastric mucous tissue and serum before and after treatment. Methods The patients（ n = 80） were randomly divided into 2 groups according to the design of randomized single blind controlled clinical trials,and treatment group was treated with Huazhuo Jiedu Fang and control group was treated with Alatan Wuwei Wan. The expression levels of Akt and c-Met in serum and gastric mucous tissue were observed in 2 groups before and after treatment. Results The expression level of Akt decreased significantly in treatment group compared with control group（ P 〈 0. 05） in serum and gastric mucous tissue,and expression level of c-Met in gastric mucous tissue decreased significantly in treatment group compared with control group（ P 〈 0. 05）. Conclusion Huazhuo Jiedu Fang can reduce significantly expression levels of Akt and c-Met in serum and gastric mucous tissue in patients with chronic erosive gastritis CEG（ syndrome of turbidity-toxin accumulating in the interior）. The effect mechanism may be that Huazhuo Jiedu Fang can regulate expression levels and activities of Akt and c-Met.