针刺联合美多芭对帕金森病小鼠脑多巴胺神经元及蛋白激酶B表达的影响

Influence of acupuncture combining madopar on dopaminergic neurons and Akt expressions in mice with Parkinson's disease

目的通过观察针刺舞蹈震颤控制区联合美多芭对帕金森病(PD)小鼠脑内蛋白激酶B(Akt)表达的影响来探讨针刺联合美多芭对多巴胺(DA)能神经元的保护作用及可能机制。方法将C57BL/6雄性小鼠随机分为正常组、模型组、美多芭组、针刺组和针刺联合美多芭(简称针美)组。应用腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)的方法建立PD小鼠模型,后3组在造模后采取不同治疗措施,造模后小鼠存活28 d。采用爬杆法观察小鼠行为学;免疫组织化学技术检测小鼠中脑黑质致密部多巴胺神经元的丢失程度及脑Akt的表达。结果 3个治疗组小鼠行为学爬杆时间均较模型组有明显缩短(P<0.05)。造模后,小鼠黑质致密部多巴胺神经元表达较正常组减少(P<0.05),其中针美组多巴胺神经元表达较模型组及美多芭组增多(P<0.05)。在黑质致密部,针美组Akt与其余各组相比,表达明显升高(P<0.05);在海马部位,针美组在CA1、CA3和CA4区的表达比模型组和美多芭组明显升高(P<0.05),在CA1和CA4的表达比针刺组有明显上升(P<0.05)。结论针刺舞蹈震颤控制区特别是再给予美多芭复合治疗可能通过调节脑磷酯酰肌醇-3-羟激酶(PI3K)/Akt通路,从而达到治疗PD的目的。

Objective To investigate the protective effect of acupuncture combining madopar on dopaminergic neurons and possible mechanism through observation on the influence of acupuncture in chorea-tremble controlled zone combining madopar on cerebral protein kinase B（ Akt） expressions in mice with Parkinson ＇s disease（ PD）. Methods Male C57BL/6 mice were randomly divided into normal group,model group,madopar group,acupuncture group（ A group） and acupuncture combining madopar group（ A ＋ M group）. The mouse model of PD was established by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（ MPTP）,and madopar group,A group and A ＋ M group were given different therapies. All mice survived for 28 d after modeling. The ethnology of mice was observed by using pole climbing method. The lost of nigra dopaminergic neurons and Akt expressions were detected by using immunohistochemistry technique. Results The time of pole climbing were significantly shorter in madopar group,A group and A ＋ M group than that in model group（ P 〈 0. 05）.After modeling,the expression of nigra dopaminergic neurons decreased in madopar group,A group and A ＋ M group than that in normal group（ P 〈 0. 05）,and increased in A ＋ M group than that in model group and madopar group（ P 〈 0. 05）. The expression of Akt in nigra compacta increased significantly in A ＋ M group than that in other groups（ P 〈 0. 05）. The expressions of Akt at zones of CA1,CA3 and CA4 in hippocampal part increased significantly in A ＋ M group than those in model group and madopar group（ P 〈 0. 05）,and the expressions at zones of CA1 and CA4 increased significantly than those in A group（ P 〈 0. 05）. Conclusion Acupuncture in chorea-tremble controlled zone,especially combining madopar then,can get a therapeutic effect on PD through possibly regulating PI3K/Akt pathway.