成纤维细胞激活蛋白与慢性乙型肝炎相关性肝病的关系

The correlation between fibroblast activation protein and chronic Hepatitis B-related liver diseases

目的通过比较乙型肝炎(乙肝)相关性肝癌、乙肝肝硬化以及慢性乙肝患者肝组织中成纤维细胞激活蛋白(FAP)水平,探讨FAP在乙肝相关性慢性肝脏疾病的表达差异及其意义。方法收集47例乙肝相关性肝癌、29例乙肝肝硬化(S4期)、22例慢性乙肝(S1~S3期)及17例因良性病变行肝切除术的肝组织,采用反转录聚合酶链反应(RT-PCR)分别检测其中FAP的表达情况;采用酶联免疫吸附(ELISA)方法检测组织及血清中FAP蛋白表达水平。结果在肝癌、肝硬化、肝炎以及对照组肝组织中,FAP mRNA表达的差异倍数值分别是4.73±1.60、1.86±1.04、0.98±0.80及1.00±0.00,四者比较差异有统计学意义(F=7.156,P=0.000);ELISA结果显示FAP蛋白在肝癌患者组织中1μg总蛋白平均含量为(1 288.28±695.82)pg/mL,而肝硬化组织为(1 176.11±677.43)pg/mL,肝炎组织为(1 044.58±389.48)pg/mL,对照组为(848.70±540.74)pg/mL,差异有统计学意义(F=3.004,P=0.034)。结论 FAP在肝炎、肝硬化、肝癌以及对照组的mRNA及蛋白表达水平存在差异,随着疾病的进展,FAP呈渐进性表达。

Objective To analyze the mRNA expression and significance of fibroblast activation protein （FAP） in HBV related hepatocellular carcinoma （HCC）, cirrhosis and chronic hepatitis. Methods We collected specimens from 47 HCC patients with surgical resection, 29 HBV related liver cirrhosis and 22 chronic hepatitis; and 17 normal liver tissues were also collected as control. Real - time PCR was performed to analyze the expression of FAP mRNA. Enzyme - linked immunosorbent assay was used to measure FAP protein concentrations. Results The mRNA expression of FAP in liver tissues from HCC, cirrhosis, hepatitis and normal controls was 4.73 ± 1.60, 1.86 ±1.04, 0. 98 ± 0. 80 and 1.00 ± 0.00 ; among which there were significant differences （ F = 7. 156, P 〈 0. 05 ）. Enzyme - linked immunosorbent assay showed that the concentrations of FAP in 1 μg total protein in liver tissues from HCC, cirrhosis, chronic hepatitis and normal controls were （ 1 288.28± 695.82 ） pg/mL, （ 1 176. 11 ± 677.43 ） pg/mL, （ 1 044. 58 ±389.48 ） pg/mL and （848.70 ± 540. 74） pg/mL, respectively. Conclusion There are significant differences in the expression of FAP mRNA among hepatitis, cirrhosis, liver cancer and normal control; as the disease progresses, the expression of FAP is progressive.