2013—2015年石家庄市HIV确证结果分析

Analysis of HIV confirmatory results in Shijiazhuang city from 2013 to 2015

目的分析石家庄市艾滋病确证实验室HIV抗体初筛阳性样本复检和确证实验结果,提高实验室HIV抗体检测能力。方法对石家庄市2013—2015年HIV抗体初筛阳性的样品,采用ELISA试剂和快诊试剂进行复检,任一复检试验阳性的样本,再用免疫印迹法(WB)进行确证实验。结果共收集2 684份初筛阳性样本,复检阳性率为65.76%,WB确证阳性率为71.16%。确证HIV-1抗体阳性病例1 256例,以男性为主(占89.49%),主要集中在26~45岁(占50.08%),性传播是最主要的传播途径(占93.71%),以同性性接触为主(占67.71%)。WB带形图多为9条带(占55.33%),全带型和次全带型占96.02%。不确定样本中完成追踪检测51人,有28人转阳,占54.90%。49.97%的复检阳性样品来自医疗机构,35.58%来自疾控中心。送检机构的确证阳性率,由高到低分别为疾控中心(81.69%)、医疗机构(74.72%)、出入境检验机构(34.85%)、采供血机构(32.28%),差异有统计学意义(P<0.05);确证病例来源,主要为术前检测和自愿咨询检测者,分别占37.73%和29.91%。结论石家庄市HIV确证检阳性率相对稳定,医疗机构主动提供艾滋病检测咨询(PITC)和艾滋病自愿检测(VCT)已逐步成为发现HIV感染者的重要方式。

Objective To analyze re-examination and confirmatory results on human immunodeficiency virus （HIV） antibod- y screening positive samples in Shijiazhuang city;to improve laboratory HIV antibody detection capacity. Methods ELISA and quick diagnosis method were used to re-examine HIV antibody screening positive samples collected from 2013 to 2015 in Shiji- azhuang city. Samples with at least one positive results from above 2 methods were subjected to confirmatory test by Western Blot （WB） analysis. Results A total of 2 684 HIV antibody screening positive samples were re-examined ,resulting re-exami- nation positive rate of 65.76%. The WB positive rate was 71.16%. A total of 1 256 HIV-1 antibody positive cases were con- firmed, mainly were males（accounted for 89.49%）, from 26 to 45 years old（accounted for 50. 08%）. Sexual transmission was major infection route（accounted for 93.71%） ,among which homosexual transmission accounted for 67. 71%. The 9-bands pat- tern was the major banding pattern （accounting for 55.33 %）, the complete banding pattern and sub-complete banding pattern accounted for 96.02% ,A total of 51 undefined cases were traced for follow-up successfully , among whom 28 were confirmed to be HIV-1 antibody positive cases, accounting for 54.90%. 49.97% of re-examined samples were collected from medical in- stitutions, while 35.58% were collected from CDC. The descending order of sample confirmatory positive rates of different sources are the CDC （81.69%）, medical institutions （74.72%）, boarder inspection agency （34.85%）, blood center （32.280%）, the difference was statistically significant （P〈0. 05）. Confirmed cases mainly were subjects taking pre-operative detection and voluntary counseling and testing, accounting for 37.73% and 29.91%, respectively. Conclusion The HIV con- firmatory positive rate of Shijiazhuang is relatively stable. Provider initiated testing and counseling （PITC） and voluntary HIV testing （VCT） gradually become important ways to identify HIV infected patients.