三种鸡胚绒膜尿囊膜移植瘤模型对比分析

Contrastive Analysis of three transplanted tumor models in chick embryo chorioallantoic membrane

目的通过比较三种不同癌细胞株在鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)上的生长情况,从中筛选最佳移植癌细胞株,建立移植瘤模型并观察生物学特性。方法分别将人肺癌A549株、舌鳞癌TCA8113株与人肝癌QGY7703株接种于7日龄鸡胚CAM上,对各自鸡胚成活率、瘤株成活、成瘤率和诱导血管生成情况等指标进行检测,并观察筛选移植瘤模型生长特性。结果与人肺癌A549株和肝癌QGY7703株接种组相比,舌鳞癌TCA8113株接种组成瘤率最高(P<0.05),为最佳移植癌细胞株,最佳接种癌细胞数量为8.0×106/个,接种后瘤体最佳生长周期为4~8 d,最佳实验时间为接种后第7天。结论筛选建立了舌鳞癌TCA-CAM移植瘤模型,为深入研究肿瘤生长生物学特性、血管生成机制、侵袭转移机制和筛选抗肿瘤药物提供了良好的实验动物模型。

Objective To compare the growth of three different cancer cell lines on chick chorioallantoic membrane（CAM）,to select the best transplanted cancer cell line for establishing a transplanted tumor model and to observe the biological characteristics.Methods The human lung cancer cell line A549,human tongue cancer cell line TCA8113 and human liver cancer cell line QGY7703 were respectively inoculated into CAM at the 7th day of age.The chick embryo survival rate,tumor survival rate,tumor formation rate and induced angiogenesis were detected and the growth characteristics of the transplanted tumor model were observed.Results Compared with the groups inoculated with A549 cells and QGY7703 cells,the tumor formation rate of TCA8113 cells was the highest（P〈0.05）,to be the best cancer cell line for transplanted tumor.The optimal inoculated number of cells was 8.0 × 106/chick embryo,the optimal growth period of the tumor was 4 ~ 8 d,and the best experiment time was 7 d after inoculation.Conclusion The TCACAM transplanted tumor model of tongue squamous cell cancer is successfully established for further study of the biological characteristics and mechanisms of tumor growth,angiogenesis,invasion and metastasis,and provide a good experimental animal model for anti-tumor drug screening.