甲状腺功能亢进时血中血管紧张素(1-7)、血管紧张素Ⅱ比值变化及其意义

The change and significance of Ang( 1-7 ) and Ang II ratio in serum of hyperthyroidism

目的探讨甲状腺功能亢进(甲亢)血中血管紧张素(1-7)[Ang(1-7)]、血管紧张素Ⅱ(AngⅡ)比值变化及其意义。方法 SD大鼠40只,随机分为实验组和对照组,每组20只。实验组采用左旋甲状腺素钠灌胃法(每天0.5 mg/kg)建立甲状腺功能亢进模型,对照组给予等容量的生理盐水。观察实验开始后第0、2、4、6、8周各组大鼠心率、收缩压、体质量变化,检测血中T3、T4、Ang(1-7)、AngⅡ水平,计算心脏/体质量比值,光镜下观察心肌组织病理学改变。结果实验第2周开始,实验组心率、收缩压高于对照组(P<0.05),体质量低于对照组(P<0.05),血T3、T4水平高于对照组(P<0.05),实验组大鼠心脏/体质量比值逐渐升高,均高于对照组(P<0.05),光镜下心肌损害程度逐渐加重。实验第4周开始,Ang(1-7)/AngⅡ比值逐渐减小,均低于对照组(P<0.05)。结论随着甲亢病情进展,血中Ang(1-7)/AngⅡ比值逐渐减小,检测Ang(l-7)/AngⅡ比值变化有助于评估甲亢致心肌损害程度。

Objective To investigate the change and significance of Ang（ 1-7 ）and AngⅡ ratio in serum of hyperthyroidism. Methods Forty SD rats were randomly divided into the experimental group and the control group, with 20 rats in each group. In the experimental group, the rat model of hyperthyroidism was established by using the method of gastric lavage with levothyroxine sodium 0.5 mg/kg/d and the control group was given equal volume of normal saline. After the experiment beginning of 2,4,6 and 8 weeks, the following indicators of the rats were detected, such as heart rate, systolic blood pressure, body weight changes, blood T3, T4, Ang （ 1-7 ）, AngⅡ level, heart / body weight ratio calculation, the pathological changes of myocardial tissue was observed under the light microscope. Results From the second week of the experiment, the heart rate, systolic blood pressure in the experimental group were significantly higher than the control group（ P 〈 0.05 ）. The body weight was significantly lower（ P 〈 0.05 ）, the serum T3, T4 levels were significantly higher in the experimental group （ P 〈 0.05 ）. The heart / body weight ratio increased gradually in the experimental group, were significantly higher than control group （ P 〈 0.05 ）. The myocardial damage degree under light microscope increased gradually. The Ang（ 1-7 ）/AngⅡ ratio gradually decreased from the fourth weeks of the experiment, which was significantly lower than that of the control group（ P 〈 0.05 ）. Conclusion With the progression of the disease, the/AngⅡ （ 1-7 ） Ang ratio in the blood of rats gradually decreases, and the change of Ang（ 1-7 ）/AngⅡ ratio is helpful to evaluate the degree of myocardial damage caused by hyperthyroidism.