新布尼亚病毒IgG抗体间接ELISA检测方法的建立

Establishment and application of indirect ELISA of IgG antibody against new bunyavirus

目的:建立基于间接ELISA法检测新布尼亚病毒Ig G抗体的血清学检测方法。方法:应用基因工程原核表达的新布尼亚病毒核蛋白,初步建立用于新布尼亚病毒Ig G抗体检测的间接ELISA法,用该方法对267例发热伴血小板减少综合征患者急性期和恢复期双份血清和198份标本进行检测,并与间接免疫荧光法的结果进行对比。结果:所建立的新布尼亚病毒Ig G抗体间接ELISA检测方法重复性好,与其他病毒无交叉反应。与间接免疫法对267例发热伴血小板减少综合征病例的实验室诊断结果有较好的一致性(Kappa=0.69),健康人血清检测阳性率为5.56%。结论:建立的间接ELISA方法适用于新布尼亚病毒感染的实验室诊断及流行病学调查。

Aim： To establish an indirect ELISA method for detecting Ig G antibody against new bunyavirus. Methods： Based on nucleoprotein gene of new bunyavirus obtained by prokaryotic expression,a qualitative indirect ELISA method for detecting Ig G antibody was set up. Using the established method,serum samples from both acute and recovery phases of 267 patients with fever thrombocytopenia and leukopenia syndrome and 198 healthy persons were tested. The results were compared with IFA. Results： The established indirect ELISA method had better repeatability,no cross reaction with other virus. There was no significant difference in results between IFA and indirect ELISA in 267 patients with fever thrombocytopenia and leukopenia syndrome（ Kappa = 0. 69）,and the positive rate in the healthy was 5. 56%. Conclusion： The indirect ELISA method for detecting Ig G antibody against new bunyavirus has been established successfully,which is suitable for laboratory diagnosis and epidemiological investigation for fever thrombocytopenia and leukopenia syndrome.