不同类型子痫前期患者胎盘组织内NFAT5、MCP-1的表达

Expressions of NFAT5 and MCP-1 in patients with different types of preeclampsia placenta tissue

目的:检测重度子痫前期患者胎盘组织中NFAT5、MCP-1的表达并探讨二者与新生儿结局的关系。方法:采用免疫组化法检测30例早发型重度子痫前期(早发型组)、30例晚发型重度子痫前期(晚发型组)及30例足月妊娠(对照组)孕妇胎盘组织中NFAT5、MCP-1蛋白的表达情况,采用RT-PCR法检测胎盘组织中NFAT5、MCP-1mRNA的表达情况,分析早发型组2种蛋白的相关性及与新生儿结局的关系。结果:3组胎盘组织中均有NFAT5和MCP-1蛋白的表达;早发型组2种因子的蛋白及mRNA表达水平均高于晚发型组(P<0.05);晚发型组与对照组比较,差异无统计学意义(P>0.05)。早发型组胎盘组织中NFAT5与MCP-1蛋白的表达呈正相关(r=0.779,P<0.001);而NFAT5和MCP-1蛋白分别与新生儿体重(r=-0.760、-0.560,P<0.05)、胎盘质量(r=-0.583、-0.464,P<0.05)呈负相关。结论:早发型子痫前期胎盘组织中NFAT5、MCP-1的表达均高于晚发型子痫前期,说明两者可能有不同的发病机制。

Aim： To investigate the expressions of NFAT5 and MCP-1 and their relationship with the perinatal outcome in patients with severe preeclampsia. Methods： The expressions of NFAT5 and MCP-1 in 30 case of early-onset preeclampsia（ EOPE group）,30 cases of late-onset preeclampsia（ LOPE group） and 30 normal control（ control group） tissue were detected by immunohistochemistry. RT-PCR was conducted to analyze the expressions of NFAT5 and MCP-1 mRNA in placental tissue. The relationship among the expressions of NFAT5 and MCP-1 protein and the perinatal outcome in EOPE group was analyzed. Results： NFAT5 and MCP-1 protein and mRNA expressions in the EOPE group were both higher than those in the LOPE group（ P〈0. 05）. However,there was no difference between the LOPE group and the control group（ P〈0. 05）. The expression of NFAT5 was positively correlated with the expression of MCP-1 in the EOPE group（ r =0. 779,P〈0. 001）. NFAT5 and MCP-1 expressions in the EOPE group was negatively correlated with neonatal weight（ r =- 0. 760,- 0. 560,P〈0. 05） and the placental weight（ r =- 0. 583,- 0. 464,P〈0. 05）. Conclusion： The expressions of NFAT5 and MCP-1 in the EOPE group are both higher than those in the LOPE group,which suggests that they may have different pathogenesis.