摘要
目的探讨过氧化氢低温等离子体灭菌器灭菌效果的监测方法。方法自制5种内径2~4 mm、长500~2 500 mm的模拟综合生物PCD,每次取2~3种模拟综合生物PCD放置在6个测试点,分3批次共进行24个全循环灭菌周期,灭菌结束后,进行生物培养并判读培养结果是否合格。结果 3批次180个测试管不合格率26.1%;144个测试点不合格率24.3%;不合格率最高的下中位置测试管与测试点不合格率分别为7.78%、7.63%;下前中位置(内径2~4 mm,长度500 mm)测试管与测试点不合格率为0;管腔内径与长度相等满载不合格率5.00%大于空载不合格率2.22%。结论本实验制作的管腔内径≥2 mm,长500 mm的模拟综合生物PCD可以对过氧化氢低温等离子体灭菌器的灭菌效果进行监测,并以此结果判定灭菌是否合格。
Objective To investigate the method for monitoring sterilization effect of low temperature hydrogen peroxide plasma sterilizer. Methods 5 kinds of simulation integrated biological PCD with 2-4 mm's inner diameter and 500-2 500 mm's long were made. 2-3 kinds of simulation integrated biological PCD were taken every time which were placed in six test points for operating 24 full circle sterilization cycle in 3 times. After the sterilization,biological cultivation had been done to see whether the interpretation results were qualified. Results Among the 180 test tubes of 3 batches,26. 1%was not qualified. 24. 3% of the 144 test points were not qualified. The unqualified rate which was the highest of the test tubes and test points in the middle of the lower layer was 7. 78% and 7. 63% respectively. The unqualified rate of the test tubes and test points in middle of the first lines in lower layer with a simulation integrated biological PCD( inner diameter 2-4 mm,length 500 mm) was 0. The unqualified rate( 5. 00%) of the full-load test tubes with the same lumen diameter and length was more than 2. 22% which was the unqualified rate of no-load test tubes. Conclusion The sterilization effect of low temperature hydrogen peroxide plasma sterilizer can be monitored by the simulation integrated biological PCD which are made in this experiment with inner diameter of 2 mm or more and 500 mm's long,and to determine whether the sterilization results are qualified.
出处
《中国消毒学杂志》
CAS
北大核心
2017年第3期236-238,241,共4页
Chinese Journal of Disinfection
关键词
模拟综合生物PCD
过氧化氢低温等离子体灭菌器
灭菌效果
监测
simulation integrated biological PCD
low temperature hydrogen peroxide plasma sterilizer
the sterilization effect
monitoring