miR-128对人黑素瘤细胞A375增殖和PTEN及p-Akt蛋白表达的影响

Effects of miR-128 on the proliferation of human melanoma cell line A375 and expression of PTEN and p-Akt

目的研究miR-128对人皮肤黑素瘤细胞系A375的增殖能力和对PTEN及p-Akt蛋白表达的影响,探讨miR-128影响黑色素细胞增殖的机制。方法将miR-128 mimics或inhibitor应用阳离子脂质体LipofectamineTM2000转染A375细胞,MTT检测转染后细胞增殖活力变化,Western blot方法检测PTEN及p-Akt蛋白表达。结果转染miR-128 mimics后,A375细胞增殖活力明显增强,p-Akt蛋白表达明显上调,PTEN蛋白表达显著降低;转染miR-128 inhibitor后,A375细胞增殖活力明显降低,p-Akt蛋白表达明显下调,PTEN蛋白表达显著升高。结论 miR-128促进人黑素瘤细胞A375增殖可能与调控PTEN及p-Akt的表达相关。

Objective To investigate the effects of miR-128 on the proliferation of human melanoma cell line A375 and expression of PTEN and p-Akt in cell line A375.Methods miR-128 mimics or inhibitor were transfected into A375 cells by lipofectamine package, the cell viability was analyzed by MTT assay, and Western blot was employed to detect the expressions of PTEN and p-Akt protein. Results miR-128 mimics significantly promoted A375 cells proliferation, markedly upregulated p-Akt expression level, but downregulated PTEN expression level in cell line A375. miR-128 inhibitor significantly inhibited A375 cells proliferation, downregulated p-Akt expression level, but upregulated PTEN level markedly cell line A375. Conclusion The promotion of A375 cells proliferation by MiR-128 might be related to the regulation of PTEN and p-Akt expressions.