抑瘤素M在狼疮鼠肾组织中的表达及意义

Expression of Oncostatin M in Kidney of MRL/lpr Mice

该文探讨了抑瘤素M(oncostatin M,OSM)在狼疮鼠肾组织中的表达及意义。以MRL/Mp J鼠作为正常对照鼠,MRL/lpr鼠随作为狼疮鼠。收集动物血、尿进行生化指标检测;收集肾组织后免疫组织化学和酶联免疫吸附实验检测OSM的表达情况;qRT-PCR和Western blot分别检测E-钙黏蛋白(E-cadherin)、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)及I型胶原(collagen I,Col I)mRNA和蛋白的表达;实时荧光定量PCR(quantitative Real-time PCR,qRT-PCR)检测纤维黏连蛋白(fibronectin,FN)mRNA的表达。结果发现,与对照鼠相比,狼疮鼠肾组织中OSM的表达明显增多,且其表达与血尿素氮、24 h尿蛋白及α-SMA、Col I和FN的表达呈正相关,而与E-cadherin的表达呈负相关。结果表明,OSM在狼疮鼠肾组织中的表达升高,且其高表达可能与狼疮鼠肾功能减退及肾小管上皮细胞转分化有一定的关系。

The aim of this study is to investigate the expression of OSM（oncostatin M） in the renal cortex of MRL/lpr mice. MRL/Mp J mice were designated as the control group and MRL/lpr mice were designated as the lupus group. The expression of OSM was detected by the methods of immunohistochemistry and enzyme linked immunosorbent assay. Western blot and quantitative Real-time PCR（qRT-PCR） analysis were used to determine the protein and mRNA expression of E-cadherin, α-SMA（α-smooth muscle actin） and Col I（collagen I）. qRTPCR analysis was used to detected the expression of fibronectin（FN） mRNA. The results suggested that the OSM expression increased in lupus mice compared with control mice. And the expression level of OSM is positively correlated with blood urea nitrogen, 24 h urinary protein and the expression level of α-SMA, Col I and FN but is negatively correlated with the expression level of E-cadherin. The results indicated that OSM expression is increased in kidney in lupus mice, which may connected with the renal dysfunction and tubular-epithelial cell transdifferentiation.