摘要
蛋白质的亚细胞定位是功能基因组学的重要研究内容。瞬时表达是一种简单快速的研究蛋白质亚细胞定位的实验技术。该实验设计了适合为本科生开设的综合实验课程。该实验项目利用在烟草叶片中瞬时表达荧光蛋白研究蛋白质的亚细胞定位。该文对实验原理、方法及教学安排等进行了详细的介绍,该实验设计为高校生物学实验教学提供了有价值的参考。
Genome sequencing has resulted in the identification of a large number of genes with unknown function. Determination of the subcellular localization of the encoded proteins is crucial for identifying their function. Transient expression is a simple and fast technique to study the subcellular localization of proteins. Here, we present an experimental course suitable for undergraduate students. In this course, students will analyze the intracellular localization of fluorescent proteins transiently expressed in tobacco leaves. We describe the experimental principle, step-by-step protocol, and teaching arrangement in detail. This experiment provides valuable references for the experimental teaching for undergraduate students.
出处
《中国细胞生物学学报》
CAS
CSCD
2017年第3期341-347,共7页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31570247
91417308
31460453)
天津市自然科学基金(批准号:14JCYBJC41200)资助的课题~~
关键词
亚细胞定位
瞬时表达
烟草
荧光蛋白
subcellular localization
transient expression
tobacco
fluorescent protein