蛋白质亚细胞定位实验课程在细胞生物学实验教学中的应用

Determination of the Subcellular Localization of Proteins as An Experimental Course in Cell Biology

蛋白质的亚细胞定位是功能基因组学的重要研究内容。瞬时表达是一种简单快速的研究蛋白质亚细胞定位的实验技术。该实验设计了适合为本科生开设的综合实验课程。该实验项目利用在烟草叶片中瞬时表达荧光蛋白研究蛋白质的亚细胞定位。该文对实验原理、方法及教学安排等进行了详细的介绍,该实验设计为高校生物学实验教学提供了有价值的参考。

Genome sequencing has resulted in the identification of a large number of genes with unknown function. Determination of the subcellular localization of the encoded proteins is crucial for identifying their function. Transient expression is a simple and fast technique to study the subcellular localization of proteins. Here, we present an experimental course suitable for undergraduate students. In this course, students will analyze the intracellular localization of fluorescent proteins transiently expressed in tobacco leaves. We describe the experimental principle, step-by-step protocol, and teaching arrangement in detail. This experiment provides valuable references for the experimental teaching for undergraduate students.